The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA

Sarah D. Ackerman, Cynthia Garcia, Xianhua Piao, David H. Gutmann, Kelly Monk

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

In the vertebrate central nervous system, myelinating oligodendrocytes are postmitotic and derive from proliferative oligodendrocyte precursor cells (OPCs). The molecular mechanisms that govern oligodendrocyte development are incompletely understood, but recent studies implicate the adhesion class of G protein-coupled receptors (aGPCRs) as important regulators of myelination. Here, we use zebrafish and mouse models to dissect the function of the aGPCR Gpr56 in oligodendrocyte development. We show that gpr56 is expressed during early stages of oligodendrocyte development. In addition, we observe a significant reduction of mature oligodendrocyte number and myelinated axons in gpr56 zebrafish mutants. This reduction results from decreased OPC proliferation, rather than increased cell death or altered neural precursor differentiation potential. Finally, we show that these functions are mediated by Gα12/13 proteins and Rho activation. Together, our data establish Gpr56 as a regulator of oligodendrocyte development.

Original languageEnglish (US)
Article number6122
JournalNature Communications
Volume6
DOIs
StatePublished - Jan 21 2015
Externally publishedYes

Fingerprint

Oligodendroglia
G-Protein-Coupled Receptors
adhesion
Adhesion
regulators
Cell proliferation
Neurology
Cell death
proteins
Chemical activation
interactions
axons
vertebrates
central nervous system
Zebrafish
death
mice
G12-G13 GTP-Binding Protein alpha Subunits
Proteins
activation

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA. / Ackerman, Sarah D.; Garcia, Cynthia; Piao, Xianhua; Gutmann, David H.; Monk, Kelly.

In: Nature Communications, Vol. 6, 6122, 21.01.2015.

Research output: Contribution to journalArticle

Ackerman, Sarah D. ; Garcia, Cynthia ; Piao, Xianhua ; Gutmann, David H. ; Monk, Kelly. / The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA. In: Nature Communications. 2015 ; Vol. 6.
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