The adaptor protein Tks5/Fish is required for podosome formation and function, and for the protease-driven invasion of cancer cells

Darren F. Seals, Eduardo F. Azucena, Ian Pass, Lia Tesfay, Rebecca Gordon, Melissa Woodrow, James H. Resau, Sara Courtneidge

Research output: Contribution to journalArticle

234 Citations (Scopus)

Abstract

Tks5/Fish is a scaffolding protein with five SH3 domains and one PX domain. In Src-transformed cells, Tks5/Fish localizes to podosomes, discrete protrusions of the ventral membrane. We generated Src-transformed cells with reduced Tks5/Fish levels. They no longer formed podosomes, did not degrade gelatin, and were poorly invasive. We detected Tks5/Fish expression in podosomes in invasive cancer cells, as well as in human breast cancer and melanoma samples. Tks5/Fish expression was also required for protease-driven matrigel invasion in human cancer cells. Finally, coexpression of Tks5/Fish and Src in epithelial cells resulted in the appearance of podosomes. Thus, Tks5/Fish appears to be required for podosome formation, for degradation of the extracellular matrix, and for invasion of some cancer cells.

Original languageEnglish (US)
Pages (from-to)155-165
Number of pages11
JournalCancer Cell
Volume7
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Fish Proteins
Peptide Hydrolases
Fishes
Neoplasms
src Homology Domains
Gelatin
Extracellular Matrix
Melanoma
Epithelial Cells
Podosomes
Breast Neoplasms
Membranes
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

Cite this

The adaptor protein Tks5/Fish is required for podosome formation and function, and for the protease-driven invasion of cancer cells. / Seals, Darren F.; Azucena, Eduardo F.; Pass, Ian; Tesfay, Lia; Gordon, Rebecca; Woodrow, Melissa; Resau, James H.; Courtneidge, Sara.

In: Cancer Cell, Vol. 7, No. 2, 02.2005, p. 155-165.

Research output: Contribution to journalArticle

Seals, Darren F. ; Azucena, Eduardo F. ; Pass, Ian ; Tesfay, Lia ; Gordon, Rebecca ; Woodrow, Melissa ; Resau, James H. ; Courtneidge, Sara. / The adaptor protein Tks5/Fish is required for podosome formation and function, and for the protease-driven invasion of cancer cells. In: Cancer Cell. 2005 ; Vol. 7, No. 2. pp. 155-165.
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