Abstract
The action of neurotensin was studied on single myenteric neurones within ganglia of the myenteric plexus isolated from the guinea-pig ileum. Drugs were applied by adding them to the perfusing Krebs solution. Extracellular recording with glass suction electrodes indicated that neurotensin (100 pM-300 nM) caused a dose-dependent excitation of about 50% of myenteric neurones; the remaining neurones were unaffected. This effect persisted in calcium-free solutions. Intracellular recording showed that a similar proportion of Type 1 myenteric neurones were depolarized by neurotensin: this was associated with an increase in membrane resistance. Type 2 cells were either depolarized or hyperpolarized by neurotension. The depolarization persisted in calcium-free solutions. The hyperpolarization disappeared in calcium-free solutions, suggesting either that the potential change itself is calcium-dependent or that it was due to release by neurotensin of a hyperpolarizing substance.
Original language | English (US) |
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Pages (from-to) | 181-186 |
Number of pages | 6 |
Journal | European Journal of Pharmacology |
Volume | 59 |
Issue number | 3-4 |
DOIs | |
State | Published - Nov 16 1979 |
Externally published | Yes |
Keywords
- Eletrophysiology
- Myenteric neurones
- Neurotensin
ASJC Scopus subject areas
- Pharmacology