The action of neurotensin on single myenteric neurones

John T. Williams, Yoshifumi Katayama, R. Alan North

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The action of neurotensin was studied on single myenteric neurones within ganglia of the myenteric plexus isolated from the guinea-pig ileum. Drugs were applied by adding them to the perfusing Krebs solution. Extracellular recording with glass suction electrodes indicated that neurotensin (100 pM-300 nM) caused a dose-dependent excitation of about 50% of myenteric neurones; the remaining neurones were unaffected. This effect persisted in calcium-free solutions. Intracellular recording showed that a similar proportion of Type 1 myenteric neurones were depolarized by neurotensin: this was associated with an increase in membrane resistance. Type 2 cells were either depolarized or hyperpolarized by neurotension. The depolarization persisted in calcium-free solutions. The hyperpolarization disappeared in calcium-free solutions, suggesting either that the potential change itself is calcium-dependent or that it was due to release by neurotensin of a hyperpolarizing substance.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalEuropean Journal of Pharmacology
Volume59
Issue number3-4
DOIs
StatePublished - Nov 16 1979

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Keywords

  • Eletrophysiology
  • Myenteric neurones
  • Neurotensin

ASJC Scopus subject areas

  • Pharmacology

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