The accumulation of IGF-I in kidneys of streptozotocin-diabetic adult rats is not associated with elevated plasma GH or IGF-I levels

Moshe Phillip, Yael Segeve, Amnon Zung, Avinoam A. Kowarski, Haim Werner, Charles Roberts, Derek LeRoith, John Ladas, Susan E. Mulroney

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nephropathy is a major complication of diabetes mellitus and is associated with expansion of the mesangium and an increase in kidney size in both humans and rats. Interestingly, early kidney enlargement occurs only in postpubertal animals, and is preceded by a significant increase in the levels of extractable renal IGF-I. This study examined the possibility that this difference is GH dependent, and that very early changes in plasma GH and/or IGF-I in the adult animal are associated with an early accumulation of renal IGF-I. Silastic jugular catheters were placed in adult (13-14 week) male Sprague-Dawley (S-D) rats for blood collection and drug injection. Serial blood samples were taken every 30 min in groups of saline control and streptozotocin (STZ) (50 μg/kg, IV) rats from 1-6 h, 9-15 h, and 24-30 h post-injection, and plasma GH profiles were determined by RIA. Renal IGF-I content was assessed following acid extraction. Following STZ, there was an immediate, step-wise reduction in peak GH levels (saline controls, 54±7 ng/ml vs 30±5 (1-6 h); 23±10 (9-15 h); and 13±3 ng/ml (24-30 h post-STZ);P

Original languageEnglish (US)
Pages (from-to)689-693
Number of pages5
JournalEndocrine
Volume3
Issue number9
DOIs
StatePublished - Sep 1995
Externally publishedYes

Fingerprint

Streptozocin
Insulin-Like Growth Factor I
Kidney
Injections
Diabetes Complications
Sprague Dawley Rats
Neck
Catheters
Control Groups
Acids
Pharmaceutical Preparations

Keywords

  • diabetes
  • growth hormone
  • IGF-I
  • kidney
  • rat

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The accumulation of IGF-I in kidneys of streptozotocin-diabetic adult rats is not associated with elevated plasma GH or IGF-I levels. / Phillip, Moshe; Segeve, Yael; Zung, Amnon; Kowarski, Avinoam A.; Werner, Haim; Roberts, Charles; LeRoith, Derek; Ladas, John; Mulroney, Susan E.

In: Endocrine, Vol. 3, No. 9, 09.1995, p. 689-693.

Research output: Contribution to journalArticle

Phillip, M, Segeve, Y, Zung, A, Kowarski, AA, Werner, H, Roberts, C, LeRoith, D, Ladas, J & Mulroney, SE 1995, 'The accumulation of IGF-I in kidneys of streptozotocin-diabetic adult rats is not associated with elevated plasma GH or IGF-I levels', Endocrine, vol. 3, no. 9, pp. 689-693. https://doi.org/10.1007/BF02746346
Phillip, Moshe ; Segeve, Yael ; Zung, Amnon ; Kowarski, Avinoam A. ; Werner, Haim ; Roberts, Charles ; LeRoith, Derek ; Ladas, John ; Mulroney, Susan E. / The accumulation of IGF-I in kidneys of streptozotocin-diabetic adult rats is not associated with elevated plasma GH or IGF-I levels. In: Endocrine. 1995 ; Vol. 3, No. 9. pp. 689-693.
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