The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails

Alberto J. Napuli, Dina Alzhanova, Catalin E. Doneanu, Douglas F. Barofsky, Eugene V. Koonin, Valerian V. Dolja

Research output: Contribution to journalArticle

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Abstract

The filamentous virion of the closterovirus Beet yellows virus (BYV) consists of a long body formed by the major capsid protein (CP) and a short tail composed of the minor capsid protein (CPm) and the virus-encoded Hsp70 homolog. By using nano-liquid chromatography-tandem mass spectrometry and biochemical analyses, we show here that the BYV 64-kDa protein (p64) is the fourth integral component of BYV virions. The N-terminal domain of p64 is exposed at the virion surface and is accessible to antibodies and mild trypsin digestion. In contrast, the C-terminal domain is embedded in the virion and is inaccessible to antibodies or trypsin. The C-terminal domain of p64 is shown to be homologous to CP and CPm. Mutation of the signature motifs of capsid proteins of filamentous RNA viruses in p64 results in the formation of tailless virions, which are unable to move from cell to cell. These results reveal the dual function of p64 in tail assembly and BYV motility and support the concept of the virion tail as a specialized device for BYV cell-to-cell movement.

Original languageEnglish (US)
Pages (from-to)2377-2384
Number of pages8
JournalJournal of Virology
Volume77
Issue number4
DOIs
StatePublished - Feb 2003

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Closterovirus
Beet yellows virus
Capsid Proteins
coat proteins
virion
Virion
tail
Trypsin
trypsin
antibodies
Antibodies
RNA Viruses
cells
Tandem Mass Spectrometry
cell movement
Liquid Chromatography
liquid chromatography
Cell Movement
Digestion
digestion

ASJC Scopus subject areas

  • Immunology

Cite this

Napuli, A. J., Alzhanova, D., Doneanu, C. E., Barofsky, D. F., Koonin, E. V., & Dolja, V. V. (2003). The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails. Journal of Virology, 77(4), 2377-2384. https://doi.org/10.1128/JVI.77.4.2377-2384.2003

The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails. / Napuli, Alberto J.; Alzhanova, Dina; Doneanu, Catalin E.; Barofsky, Douglas F.; Koonin, Eugene V.; Dolja, Valerian V.

In: Journal of Virology, Vol. 77, No. 4, 02.2003, p. 2377-2384.

Research output: Contribution to journalArticle

Napuli, AJ, Alzhanova, D, Doneanu, CE, Barofsky, DF, Koonin, EV & Dolja, VV 2003, 'The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails', Journal of Virology, vol. 77, no. 4, pp. 2377-2384. https://doi.org/10.1128/JVI.77.4.2377-2384.2003
Napuli, Alberto J. ; Alzhanova, Dina ; Doneanu, Catalin E. ; Barofsky, Douglas F. ; Koonin, Eugene V. ; Dolja, Valerian V. / The 64-kilodalton capsid protein homolog of Beet yellows virus is required for assembly of virion tails. In: Journal of Virology. 2003 ; Vol. 77, No. 4. pp. 2377-2384.
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