The 5' flanking region of the rat β3-adrenergic receptor gene: Divergence with the human gene and implications for species-specific gene expression

Julie A. Brown, Curtis A. Machida

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

β3-adrenergic receptor mRNAs exhibit species-specific expression (human vs. rodent) in distinct anatomical regions and appear to be expressed abundantly within rodent adipose tissue, but only at low levels within corresponding human tissues. In order to determine the genetic basis of the differential expression of the rat and human β3-adrenergic receptor genes, we cloned and sequenced the rat gene and compared the 5' flanking regions of the two genes to identify potential discriminators in transcriptional regulation. We have found that the rat and human β3-adrenergic receptor 5' flanking regions are only 67% similar, unlike the close sequence similarity observed between the coding blocks (> 90%) and also observed between species for the 5' flanking regions of other βadrenergic receptor subtype genes (> 90%). In addition, the rat β3-adrenergic receptor gene lacks the four potential cAMP responsive elements identified within the 5' flanking region of the human receptor gene. The striking divergence in regulatory sequences between the rat and human β3-adrenergic receptor genes may potentially explain the differences in species-specific expression and tissue localization of the rat and human receptor mRNAs.

Original languageEnglish (US)
Pages (from-to)319-324
Number of pages6
JournalMitochondrial DNA
Volume4
Issue number5
DOIs
StatePublished - Jan 1 1994

Keywords

  • Adrenergic receptors
  • Promoter
  • Species-specific expression
  • Transcriptional regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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