Testing the performance of a prototype lateral flow device using bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in high-risk patients

Caroline G. Castillo, Carol A. Kauffman, Jingyi Zhai, Hui Jiang, Stephanie M. Agozino, Marisa H. Miceli

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The diagnosis of invasive pulmonary aspergillosis (IPA) increasingly relies on non-culture-based biomarkers in bronchoalveolar lavage (BAL) fluid. The Aspergillus lateral flow device (LFD) is a rapid immunoassay that uses a novel Aspergillus monoclonal antibody to gain specificity. The objective of the study is to compare specificity and sensitivity of the prototype LFD and the galactomannan (GM) enzyme immunoassay in BAL fluid in high-risk patients. A total of 114 BAL samples from 106 patients at high risk for IPA were studied: 8 patients had proven/probable IPA, 16 had possible IPA and 82 did not have IPA. In patients with proven/probable IPA, specificity of LFD was 94% and GM was 89%; sensitivity of LFD was 38% and GM was 75%. Negative predictive value (NPV) for LFD was 94% and for GM was 98%; positive predictive value (PPV) was 38% for both tests. The use of anti-mould prophylaxis did not affect specificity but resulted in decreased NPV of both LFD and GM. Union and intersection analysis showed no improvement in the performance by using both tests. Among patients at risk for IPA, the diagnostic performance of LFD and GM in BAL fluid appears comparable; specificity is high, but sensitivity of both LFD and GM is poor.

Original languageEnglish (US)
Pages (from-to)4-10
Number of pages7
JournalMycoses
Volume61
Issue number1
DOIs
StatePublished - Jan 2018
Externally publishedYes

Keywords

  • bronchoalveolar lavage
  • diagnostic test
  • invasive aspergillosis
  • lateral flow device
  • pulmonary aspergillosis

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

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