Testicular adenosine 3':5' monophosphate dependent protein kinase. Regulation by follicle stimulating hormone

A. R. Means, E. MacDougall, T. R. Soderling, J. D. Corbin

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    Abstract

    Incubation of testis seminiferous tubules with follicle stimulating hormone (FSH) results within 5 min in an activation of soluble adenosine 3':5' monophosphate (cAMP) dependent protein kinase, as indicated by a conversion of the inactive holoenzyme to the active catalytic subunit. Maximal stimulation (3 fold) is achieved by 20 min. Moreover, this increased kinase activity can be directly correlated with increased intracellular accumulation of cAMP. Activation of protein kinase in isolated tubules is specific for FSH and is dependent upon time and temperature of incubation. This response to FSH is also dependent upon the age of the animals and disappears at approximately 30 days. However, sensitivity to gonadotrophin can be restored in adult animals by hypophysectomy or by addition of 1 methyl 3 isobutylxanthine to the incubation medium. These results suggest that the appearance of an active phosphodiesterase may be responsible for the decreased response to FSH during spermatogenesis. Increased protein kinase activity in response to the continued presence of FSH exhibits a half life time of 2 to 4 hr. Bound FSH can be recovered following treatment of the tissue of acid pH and this hormone retains the ability to activate protein kinase in fresh tissue suggesting that FSH may not be degraded while attached to testicular receptors. Excellent temporal correlation exists between binding of FSH and activation of protein kinase. Maximal enzyme activation occurs at a lower concentration of FSH than is necessary to saturate testicular binding sites.

    Original languageEnglish (US)
    Pages (from-to)1231-1238
    Number of pages8
    JournalJournal of Biological Chemistry
    Volume249
    Issue number4
    StatePublished - Jan 1 1974

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    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Means, A. R., MacDougall, E., Soderling, T. R., & Corbin, J. D. (1974). Testicular adenosine 3':5' monophosphate dependent protein kinase. Regulation by follicle stimulating hormone. Journal of Biological Chemistry, 249(4), 1231-1238.