Tertiary structure of plant RuBisCO

Domains and their contacts

Michael Chapman, Se Won Suh, Paul M G Curmi, Duilio Cascio, Ward W. Smith, David S. Eisenberg

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The three-dimensional structure of ribulose-1,5-biphosphate carboxylase-oxygenase (RuBisCO), has been determined at 2.6 Å resolution. This enzyme initiates photosynthesis by combining carbon dioxide with ribulose bisphosphate to form two molecules of 3-phosphoglycerate. In plants, RuBisCO is built from eight large (L) and eight small (S) polypeptide chains, or subunits. Both S chains and the NH2-terminal domain (N) of L are antiparallel β, "open-face-sandwich" domains with four-stranded β sheets and flanking α helices. The main domain (B) of L is an α/β barrel containing most of the catalytic residues. The active site is in a pocket at the opening of the barrel that is partly covered by the N domain of a neighboring L chain. The domain contacts of the molecule and its conserved residues are discussed in terms of this structure.

Original languageEnglish (US)
Pages (from-to)71-74
Number of pages4
JournalScience
Volume241
Issue number4861
StatePublished - 1988
Externally publishedYes

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Ribulose-Bisphosphate Carboxylase
Plant Structures
Photosynthesis
Carbon Dioxide
Catalytic Domain
Peptides
Enzymes
3-phosphoglycerate
ribulose

ASJC Scopus subject areas

  • General

Cite this

Chapman, M., Suh, S. W., Curmi, P. M. G., Cascio, D., Smith, W. W., & Eisenberg, D. S. (1988). Tertiary structure of plant RuBisCO: Domains and their contacts. Science, 241(4861), 71-74.

Tertiary structure of plant RuBisCO : Domains and their contacts. / Chapman, Michael; Suh, Se Won; Curmi, Paul M G; Cascio, Duilio; Smith, Ward W.; Eisenberg, David S.

In: Science, Vol. 241, No. 4861, 1988, p. 71-74.

Research output: Contribution to journalArticle

Chapman, M, Suh, SW, Curmi, PMG, Cascio, D, Smith, WW & Eisenberg, DS 1988, 'Tertiary structure of plant RuBisCO: Domains and their contacts', Science, vol. 241, no. 4861, pp. 71-74.
Chapman M, Suh SW, Curmi PMG, Cascio D, Smith WW, Eisenberg DS. Tertiary structure of plant RuBisCO: Domains and their contacts. Science. 1988;241(4861):71-74.
Chapman, Michael ; Suh, Se Won ; Curmi, Paul M G ; Cascio, Duilio ; Smith, Ward W. ; Eisenberg, David S. / Tertiary structure of plant RuBisCO : Domains and their contacts. In: Science. 1988 ; Vol. 241, No. 4861. pp. 71-74.
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