Temporal relationships between endometrial RNA polymerase activities and steroid hormone receptors following estradiol administration during the midluteal phase of the ovine estrous cycle

Mary Zelinski, F. Stormshak

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5 Scopus citations

Abstract

The relationships of estradiol-induced changes in nuclear-bound estrogen and RNA polymerase activities in the ovine endometrium during the midluteal phase of the estrous cycle were studied. Concomitantly, changes in cytoplasmic progesterone receptors were also monitored. Mature ewes received a single i.m. injection of 500 μg estradiol-17β on each of Days 11 and 12 of the cycle and were necropsied at 0, 1, 6, 12, and 24 hr following the second injection of hormone. Activities of RNA polymerase I and II were determined by [3H]-UTP incorporation into RNA during incubation of endometrial nuclei in the absence and presence of α-amanitin, respectively. Total nuclear and cytoplasmic steroid receptors were estimated by exchange assay using [3H]-progestin (R5020) and [3H]-estradiol. Treatment of ewes with estradiol resulted in a transient increase in the concentration of neclear-bound estradiol at 1 hr (P

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalBiology of Reproduction
Volume24
Issue number1
Publication statusPublished - 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

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