Temporal modulation of cytokine expression following focal cerebral ischemia in mice

Jennifer K. Hill, Lisa Gunion-Rinker, Doris Kulhanek, Nikola Lessov, Songte Kim, Wayne M. Clark, Michael P. Dixon, Rae Nishi, Mary P. Stenzel-Poore, Felix P. Eckenstein

Research output: Contribution to journalArticlepeer-review

105 Scopus citations


There is increasing evidence that the inflammatory response plays an important role in CNS ischemia. The murine model of focal ischemia, however, remains incompletely characterized. In this study we examined expression of several cytokines and the vascular adhesion molecule E-selectin, in order to characterize the molecular events following stroke in the C57BL/6J mouse. Using a multi-probe RNAse protection assay (RPA), mRNA for 19 cytokines was analyzed following permanent and transient occlusion of the middle cerebral artery in mice. In addition, samples from the same mice were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate E- selectin mRNA expression levels. Several cytokine mRNAs showed a similar expression pattern in both permanent and transient CNS ischemia while others showed a temporal expression pattern that was dependent on the type of stroke. For both models, mRNA levels of TNFα rose early (4 h) followed by IL-6 (10-18 h) and a comparatively late increase (96 h) in TGFβ1. IL-1α, IL-1β and IL-1ra levels showed a model dependent shift in temporal expression. Reperfusion appeared to delay the induction of these cytokines. Temporal changes in cytokine mRNA expression in the mouse CNS occur following ischemic damage. Our findings demonstrate the utility and power of multi- probe RPA for evaluation of changes in cytokine mRNA levels. Moreover, this study is, to our knowledge the first to show temporal changes in cytokine mRNA in mouse cerebral ischemia, forming a basis for further exploration of the roles of these cytokines in modulating ischemic neuronal damage in this model.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalBrain research
Issue number1-2
StatePublished - Feb 27 1999


  • Cerebral ischemia
  • Cytokine
  • E-selectin
  • Mouse

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


Dive into the research topics of 'Temporal modulation of cytokine expression following focal cerebral ischemia in mice'. Together they form a unique fingerprint.

Cite this