Temporal modulation of cytokine expression following focal cerebral ischemia in mice

Jennifer K. Hill, Lisa Gunion-Rinker, Doris Kulhanek, Nikola Lessov, Songte Kim, Wayne Clark, Michael P. Dixon, Rae Nishi, Mary Stenzel-Poore, Felix P. Eckenstein

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

There is increasing evidence that the inflammatory response plays an important role in CNS ischemia. The murine model of focal ischemia, however, remains incompletely characterized. In this study we examined expression of several cytokines and the vascular adhesion molecule E-selectin, in order to characterize the molecular events following stroke in the C57BL/6J mouse. Using a multi-probe RNAse protection assay (RPA), mRNA for 19 cytokines was analyzed following permanent and transient occlusion of the middle cerebral artery in mice. In addition, samples from the same mice were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate E- selectin mRNA expression levels. Several cytokine mRNAs showed a similar expression pattern in both permanent and transient CNS ischemia while others showed a temporal expression pattern that was dependent on the type of stroke. For both models, mRNA levels of TNFα rose early (4 h) followed by IL-6 (10-18 h) and a comparatively late increase (96 h) in TGFβ1. IL-1α, IL-1β and IL-1ra levels showed a model dependent shift in temporal expression. Reperfusion appeared to delay the induction of these cytokines. Temporal changes in cytokine mRNA expression in the mouse CNS occur following ischemic damage. Our findings demonstrate the utility and power of multi- probe RPA for evaluation of changes in cytokine mRNA levels. Moreover, this study is, to our knowledge the first to show temporal changes in cytokine mRNA in mouse cerebral ischemia, forming a basis for further exploration of the roles of these cytokines in modulating ischemic neuronal damage in this model.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalBrain Research
Volume820
Issue number1-2
DOIs
StatePublished - Feb 27 1999

Fingerprint

Brain Ischemia
Cytokines
Messenger RNA
E-Selectin
Ischemia
Interleukin-1
Stroke
Interleukin 1 Receptor Antagonist Protein
Middle Cerebral Artery Infarction
Reverse Transcriptase Polymerase Chain Reaction
Inbred C57BL Mouse
Interleukin-10
Reperfusion
Blood Vessels
Interleukin-6

Keywords

  • Cerebral ischemia
  • Cytokine
  • E-selectin
  • Mouse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hill, J. K., Gunion-Rinker, L., Kulhanek, D., Lessov, N., Kim, S., Clark, W., ... Eckenstein, F. P. (1999). Temporal modulation of cytokine expression following focal cerebral ischemia in mice. Brain Research, 820(1-2), 45-54. https://doi.org/10.1016/S0006-8993(98)01140-8

Temporal modulation of cytokine expression following focal cerebral ischemia in mice. / Hill, Jennifer K.; Gunion-Rinker, Lisa; Kulhanek, Doris; Lessov, Nikola; Kim, Songte; Clark, Wayne; Dixon, Michael P.; Nishi, Rae; Stenzel-Poore, Mary; Eckenstein, Felix P.

In: Brain Research, Vol. 820, No. 1-2, 27.02.1999, p. 45-54.

Research output: Contribution to journalArticle

Hill, JK, Gunion-Rinker, L, Kulhanek, D, Lessov, N, Kim, S, Clark, W, Dixon, MP, Nishi, R, Stenzel-Poore, M & Eckenstein, FP 1999, 'Temporal modulation of cytokine expression following focal cerebral ischemia in mice', Brain Research, vol. 820, no. 1-2, pp. 45-54. https://doi.org/10.1016/S0006-8993(98)01140-8
Hill, Jennifer K. ; Gunion-Rinker, Lisa ; Kulhanek, Doris ; Lessov, Nikola ; Kim, Songte ; Clark, Wayne ; Dixon, Michael P. ; Nishi, Rae ; Stenzel-Poore, Mary ; Eckenstein, Felix P. / Temporal modulation of cytokine expression following focal cerebral ischemia in mice. In: Brain Research. 1999 ; Vol. 820, No. 1-2. pp. 45-54.
@article{251e12d34549413dbabb014e03d2a644,
title = "Temporal modulation of cytokine expression following focal cerebral ischemia in mice",
abstract = "There is increasing evidence that the inflammatory response plays an important role in CNS ischemia. The murine model of focal ischemia, however, remains incompletely characterized. In this study we examined expression of several cytokines and the vascular adhesion molecule E-selectin, in order to characterize the molecular events following stroke in the C57BL/6J mouse. Using a multi-probe RNAse protection assay (RPA), mRNA for 19 cytokines was analyzed following permanent and transient occlusion of the middle cerebral artery in mice. In addition, samples from the same mice were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate E- selectin mRNA expression levels. Several cytokine mRNAs showed a similar expression pattern in both permanent and transient CNS ischemia while others showed a temporal expression pattern that was dependent on the type of stroke. For both models, mRNA levels of TNFα rose early (4 h) followed by IL-6 (10-18 h) and a comparatively late increase (96 h) in TGFβ1. IL-1α, IL-1β and IL-1ra levels showed a model dependent shift in temporal expression. Reperfusion appeared to delay the induction of these cytokines. Temporal changes in cytokine mRNA expression in the mouse CNS occur following ischemic damage. Our findings demonstrate the utility and power of multi- probe RPA for evaluation of changes in cytokine mRNA levels. Moreover, this study is, to our knowledge the first to show temporal changes in cytokine mRNA in mouse cerebral ischemia, forming a basis for further exploration of the roles of these cytokines in modulating ischemic neuronal damage in this model.",
keywords = "Cerebral ischemia, Cytokine, E-selectin, Mouse",
author = "Hill, {Jennifer K.} and Lisa Gunion-Rinker and Doris Kulhanek and Nikola Lessov and Songte Kim and Wayne Clark and Dixon, {Michael P.} and Rae Nishi and Mary Stenzel-Poore and Eckenstein, {Felix P.}",
year = "1999",
month = "2",
day = "27",
doi = "10.1016/S0006-8993(98)01140-8",
language = "English (US)",
volume = "820",
pages = "45--54",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Temporal modulation of cytokine expression following focal cerebral ischemia in mice

AU - Hill, Jennifer K.

AU - Gunion-Rinker, Lisa

AU - Kulhanek, Doris

AU - Lessov, Nikola

AU - Kim, Songte

AU - Clark, Wayne

AU - Dixon, Michael P.

AU - Nishi, Rae

AU - Stenzel-Poore, Mary

AU - Eckenstein, Felix P.

PY - 1999/2/27

Y1 - 1999/2/27

N2 - There is increasing evidence that the inflammatory response plays an important role in CNS ischemia. The murine model of focal ischemia, however, remains incompletely characterized. In this study we examined expression of several cytokines and the vascular adhesion molecule E-selectin, in order to characterize the molecular events following stroke in the C57BL/6J mouse. Using a multi-probe RNAse protection assay (RPA), mRNA for 19 cytokines was analyzed following permanent and transient occlusion of the middle cerebral artery in mice. In addition, samples from the same mice were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate E- selectin mRNA expression levels. Several cytokine mRNAs showed a similar expression pattern in both permanent and transient CNS ischemia while others showed a temporal expression pattern that was dependent on the type of stroke. For both models, mRNA levels of TNFα rose early (4 h) followed by IL-6 (10-18 h) and a comparatively late increase (96 h) in TGFβ1. IL-1α, IL-1β and IL-1ra levels showed a model dependent shift in temporal expression. Reperfusion appeared to delay the induction of these cytokines. Temporal changes in cytokine mRNA expression in the mouse CNS occur following ischemic damage. Our findings demonstrate the utility and power of multi- probe RPA for evaluation of changes in cytokine mRNA levels. Moreover, this study is, to our knowledge the first to show temporal changes in cytokine mRNA in mouse cerebral ischemia, forming a basis for further exploration of the roles of these cytokines in modulating ischemic neuronal damage in this model.

AB - There is increasing evidence that the inflammatory response plays an important role in CNS ischemia. The murine model of focal ischemia, however, remains incompletely characterized. In this study we examined expression of several cytokines and the vascular adhesion molecule E-selectin, in order to characterize the molecular events following stroke in the C57BL/6J mouse. Using a multi-probe RNAse protection assay (RPA), mRNA for 19 cytokines was analyzed following permanent and transient occlusion of the middle cerebral artery in mice. In addition, samples from the same mice were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate E- selectin mRNA expression levels. Several cytokine mRNAs showed a similar expression pattern in both permanent and transient CNS ischemia while others showed a temporal expression pattern that was dependent on the type of stroke. For both models, mRNA levels of TNFα rose early (4 h) followed by IL-6 (10-18 h) and a comparatively late increase (96 h) in TGFβ1. IL-1α, IL-1β and IL-1ra levels showed a model dependent shift in temporal expression. Reperfusion appeared to delay the induction of these cytokines. Temporal changes in cytokine mRNA expression in the mouse CNS occur following ischemic damage. Our findings demonstrate the utility and power of multi- probe RPA for evaluation of changes in cytokine mRNA levels. Moreover, this study is, to our knowledge the first to show temporal changes in cytokine mRNA in mouse cerebral ischemia, forming a basis for further exploration of the roles of these cytokines in modulating ischemic neuronal damage in this model.

KW - Cerebral ischemia

KW - Cytokine

KW - E-selectin

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=0033608436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033608436&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(98)01140-8

DO - 10.1016/S0006-8993(98)01140-8

M3 - Article

C2 - 10023029

AN - SCOPUS:0033608436

VL - 820

SP - 45

EP - 54

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -