Temporal effects of prolactin on casein kinase activity, casein synthesis and casein mRNA accumulation in mouse mammary gland explants

J. A. Rillema, S. M. Smolinski, G. Fan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Studies were carried out to investigate the mechanism by which prolactin stimulates casein synthesis in cultured mouse mammary tissues. The onset of the prolactin stimulation of casein synthesis occurs 8-10 hours after prolactin addition to cultured tissues; a maximum effect is evoked by 16 hours. A similar time-course of response to prolactin was observed when the tissue accumulation of the mRNA for β-casein was determined. At 16 hours, however, prolactin had no effect on tissue-specific casein kinase activity which is associated with the Golgi apparatus. These studies suggest that the tissue content of casein mRNA is causally related to the onset of the prolactin stimulation of casein synthesis in cultured mouse tissues. In contrast, the activity of the kinase that catalyzes the phosphorylation of the caseins is not altered by prolactin and thus apparently is not a contributing factor to the early prolactin effect on casein synthesis.

Original languageEnglish (US)
Pages (from-to)367-370
Number of pages4
JournalHormone and Metabolic Research
Volume26
Issue number8
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • Casein kinase
  • Casein mRNA
  • Casein synthesis
  • Galactosyl transferase
  • Mammary gland
  • Prolactin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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