Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

Nicole L. Diggins, Lindsey B. Crawford, Hillary M. Struthers, Lauren M. Hook, Igor Landais, Rebecca L. Skalsky, Meaghan H. Hancock

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level by binding to sites within the 3′ untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to detect, express and inhibit miRNAs, as well as methods used to identify and validate their targets, specifically with respect to the miRNAs encoded by human cytomegalovirus.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages301-342
Number of pages42
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2244
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Human cytomegalovirus (HCMV)
  • Locked nucleic acids (LNA)
  • Luciferase assay
  • Northern Blot
  • Photoactivatable ribonucleoside-enhanced cross-linking and Immunoprecipitation (PAR CLIP)
  • RISC-immunoprecipitation (RISC-IP)
  • Stem-loop real-time PCR
  • microRNA (miRNA)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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