Techniques for characterizing cytomegalovirus-encoded miRNAs

Lauren M. Hook, Igor Landais, Meaghan H. Hancock, Jay A. Nelson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level, by binding to sites within the 3ʹ untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to express, detect, and inhibit miRNAs as well as methods used to identify their targets.

Original languageEnglish (US)
Pages (from-to)239-265
Number of pages27
JournalMethods in Molecular Biology
Volume1119
DOIs
StatePublished - Jan 1 2014

Keywords

  • Locked nucleic acids (LNA)
  • Luciferase assay
  • MicroRNA (miRNA)
  • Northern blot
  • RISC immunoprecipitation (RISC-IP)
  • Stem-loop real-time PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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