Technical advance: Liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease

Benjamin J. Burwitz, Jason S. Reed, Katherine B. Hammond, Merete A. Ohme, Shannon L. Planer, Alfred W. Legasse, Adam J. Ericsen, Yoram Richter, Gershon Golomb, Jonah B. Sacha

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Nonhuman primates are critical animal models for the study of human disorders and disease and offer a platform to assess the role of immune cells in pathogenesis via depletion of specific cellular subsets. However, this model is currently hindered by the lack of reagents that safely and specifically ablate myeloid cells of the monocyte/macrophage Lin. Given the central importance of macrophages in homeostasis and host immunity, development of a macrophage-depletion technique in nonhuman primates would open new avenues of research. Here, using LA at i.v. doses as low as 0.1 mg/kg, we show a >50% transient depletion of circulating monocytes and tissue-resident macrophages in RMs by an 11-color flow cytometric analysis. Diminution of monocytes was followed rapidly by emigration of monocytes from the bone marrow, leading to a rebound of monocytes to baseline levels. Importantly, LA was well-tolerated, as no adverse effects or changes in gross organ function were observed during depletion. These results advance the ex vivo study of myeloid cells by flow cytometry and pave the way for in vivo studies of monocyte/macrophage biology in non-human primate models of human disease.

Original languageEnglish (US)
Pages (from-to)491-501
Number of pages11
JournalJournal of Leukocyte Biology
Volume96
Issue number3
DOIs
StatePublished - Sep 2014

Keywords

  • Bisphosphonates
  • Myeloid cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Fingerprint Dive into the research topics of 'Technical advance: Liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease'. Together they form a unique fingerprint.

Cite this