TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum

Gerco Hassink, Marjolein Kikkert, Sjaak Van Voorden, Shiow Ju Lee, Robbert Spaapen, Theo Van Laar, Catherine S. Coleman, Eric Bartee, Klaus Frueh, Vincent Chau, Emmanuel Wiertz

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

In the present study, the human TEB4 is identified as a novel ER (endoplasmic reticulum)-resident ubiquitin ligase. TEB4 has homologues in many species and has a number of remarkable properties. TEB4 contains a conserved RING (really interesting new gene) finger and 13 predicted transmembrane domains. The RING finger of TEB4 and its homologues is situated at the N-terminus and has the unconventional C4HC3 configuration. The N-terminus of TEB4 is located in the cytosol. We show that the isolated TEB4 RING domain catalyses ubiquitin ligation in vitro in a reaction that is ubiquitin Lys 48-specific and involves UBC7 (ubiguitin-conjugating enzyme 7). These properties are reminiscent of E3 enzymes, which are involved in ER-associated protein degradation. TEB4 is an ER degradation substrate itself, promoting its own degradation in a RING finger- and proteasome-dependent manner.

Original languageEnglish (US)
Pages (from-to)647-655
Number of pages9
JournalBiochemical Journal
Volume388
Issue number2
DOIs
StatePublished - Jun 1 2005

Fingerprint

Ligases
Ubiquitin
Endoplasmic Reticulum
Genes
Degradation
Endoplasmic Reticulum-Associated Degradation
Proteasome Endopeptidase Complex
Enzymes
Cytosol
Proteolysis
Fingers
Ligation
Substrates
Proteins

Keywords

  • E3 ubiquitin protein ligase
  • Endoplasmic reticulum-associated degradation
  • Proteasome
  • RING finger
  • TEB4
  • Ubiquitin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hassink, G., Kikkert, M., Van Voorden, S., Lee, S. J., Spaapen, R., Van Laar, T., ... Wiertz, E. (2005). TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum. Biochemical Journal, 388(2), 647-655. https://doi.org/10.1042/BJ20041241

TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum. / Hassink, Gerco; Kikkert, Marjolein; Van Voorden, Sjaak; Lee, Shiow Ju; Spaapen, Robbert; Van Laar, Theo; Coleman, Catherine S.; Bartee, Eric; Frueh, Klaus; Chau, Vincent; Wiertz, Emmanuel.

In: Biochemical Journal, Vol. 388, No. 2, 01.06.2005, p. 647-655.

Research output: Contribution to journalArticle

Hassink, G, Kikkert, M, Van Voorden, S, Lee, SJ, Spaapen, R, Van Laar, T, Coleman, CS, Bartee, E, Frueh, K, Chau, V & Wiertz, E 2005, 'TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum', Biochemical Journal, vol. 388, no. 2, pp. 647-655. https://doi.org/10.1042/BJ20041241
Hassink G, Kikkert M, Van Voorden S, Lee SJ, Spaapen R, Van Laar T et al. TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum. Biochemical Journal. 2005 Jun 1;388(2):647-655. https://doi.org/10.1042/BJ20041241
Hassink, Gerco ; Kikkert, Marjolein ; Van Voorden, Sjaak ; Lee, Shiow Ju ; Spaapen, Robbert ; Van Laar, Theo ; Coleman, Catherine S. ; Bartee, Eric ; Frueh, Klaus ; Chau, Vincent ; Wiertz, Emmanuel. / TEB4 is a C4HC3 RING finger-containing ubiquitin ligase of the endoplasmic reticulum. In: Biochemical Journal. 2005 ; Vol. 388, No. 2. pp. 647-655.
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