Targeted protein degradation by Salmonella under phagosome-mimicking culture conditions investigated using comparative peptidomics

Nathan P. Manes, Jean Gustin, Joanne Rue, Heather M. Mottaz, Samuel O. Purvine, Angela D. Norbeck, Matthew E. Monroe, Jennifer S D Zimmer, Thomas O. Metz, Joshua N. Adkins, Richard D. Smith, Fred Heffron

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The pathogen Salmonella enterica is known to cause both food poisoning and typhoid fever. Because of the emergence of antibiotic-resistant isolates and the threat of bioterrorism (e.g. contamination of the food supply), there is a growing need to study this bacterium. In this investigation, comparative peptidomics was used to study S. enterica serovar Typhimurium cultured in either a rich medium or in an acidic, low magnesium, and minimal nutrient medium designed to roughly mimic the macrophage phagosomal environment (within which Salmonella are known to survive). Native peptides from cleared cell lysates were enriched by using isopropanol extraction and analyzed by using both LC-MS/MS and LC-FTICR-MS. We identified and quantified 5,163 peptides originating from 682 proteins, and the data clearly indicated that compared with Salmonella cultured in the rich medium, cells cultured in the phagosome-mimicking medium had dramatically higher abundances of a wide variety of protein degradation products, especially from ribosomal proteins. Salmonella from the same cultures were also analyzed using traditional, bottom-up proteomic methods, and when the peptidomics and proteomics data were analyzed together, two clusters of proteins targeted for proteolysis were tentatively identified. Possible roles of targeted proteolysis by phagocytosed Salmonella are discussed.

Original languageEnglish (US)
Pages (from-to)717-727
Number of pages11
JournalMolecular and Cellular Proteomics
Volume6
Issue number4
DOIs
StatePublished - Apr 2007

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Phagosomes
Salmonella
Proteolysis
Degradation
Salmonella enterica
Proteomics
Proteins
Bioterrorism
Peptides
Foodborne Diseases
2-Propanol
Food Supply
Ribosomal Proteins
Typhoid Fever
Food supply
Phagocytosis
Magnesium
Macrophages
Pathogens
Cultured Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Targeted protein degradation by Salmonella under phagosome-mimicking culture conditions investigated using comparative peptidomics. / Manes, Nathan P.; Gustin, Jean; Rue, Joanne; Mottaz, Heather M.; Purvine, Samuel O.; Norbeck, Angela D.; Monroe, Matthew E.; Zimmer, Jennifer S D; Metz, Thomas O.; Adkins, Joshua N.; Smith, Richard D.; Heffron, Fred.

In: Molecular and Cellular Proteomics, Vol. 6, No. 4, 04.2007, p. 717-727.

Research output: Contribution to journalArticle

Manes, NP, Gustin, J, Rue, J, Mottaz, HM, Purvine, SO, Norbeck, AD, Monroe, ME, Zimmer, JSD, Metz, TO, Adkins, JN, Smith, RD & Heffron, F 2007, 'Targeted protein degradation by Salmonella under phagosome-mimicking culture conditions investigated using comparative peptidomics', Molecular and Cellular Proteomics, vol. 6, no. 4, pp. 717-727. https://doi.org/10.1074/mcp.M600282-MCP200
Manes, Nathan P. ; Gustin, Jean ; Rue, Joanne ; Mottaz, Heather M. ; Purvine, Samuel O. ; Norbeck, Angela D. ; Monroe, Matthew E. ; Zimmer, Jennifer S D ; Metz, Thomas O. ; Adkins, Joshua N. ; Smith, Richard D. ; Heffron, Fred. / Targeted protein degradation by Salmonella under phagosome-mimicking culture conditions investigated using comparative peptidomics. In: Molecular and Cellular Proteomics. 2007 ; Vol. 6, No. 4. pp. 717-727.
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