Targeted gene silencing to induce permanent sterility

Gregory Dissen, Alejandro Lomniczi, R. L. Boudreau, Y. H. Chen, B. L. Davidson, Sergio Ojeda

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    A non-surgical method to induce sterility would be a useful tool to control feral populations of animals. Our laboratories have experience with approaches aimed at targeting brain cells in vivo with vehicles that deliver a payload of either inhibitory RNAs or genes intended to correct cellular dysfunction. A combination/modification of these methods may provide a useful framework for the design of approaches that can be used to sterilize cats and dogs. For this approach to succeed, it has to meet several conditions: it needs to target a gene essential for fertility. It must involve a method that can selectively silence the gene of interest. It also needs to deliver the silencing agent via a minimally invasive method. Finally, the silencing effect needs to be sustained for many years, so that expansion of the targeted population can be effectively prevented. In this article, we discuss this subject and provide a succinct account of our previous experience with: (i) molecular reagents able to disrupt reproductive cyclicity when delivered to regions of the brain involved in the control of reproduction and (ii) molecular reagents able to ameliorate neuronal disease when delivered systemically using a novel approach of gene therapy.

    Original languageEnglish (US)
    Pages (from-to)228-232
    Number of pages5
    JournalReproduction in Domestic Animals
    Volume47
    Issue numberSUPPL.4
    DOIs
    StatePublished - Aug 2012

    Fingerprint

    Gene Silencing
    gene silencing
    Infertility
    brain
    gene therapy
    Essential Genes
    Brain
    Periodicity
    methodology
    periodicity
    Genetic Therapy
    Population
    Genes
    Reproduction
    Fertility
    Cats
    genes
    Dogs
    RNA
    cats

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Biotechnology
    • Endocrinology

    Cite this

    Targeted gene silencing to induce permanent sterility. / Dissen, Gregory; Lomniczi, Alejandro; Boudreau, R. L.; Chen, Y. H.; Davidson, B. L.; Ojeda, Sergio.

    In: Reproduction in Domestic Animals, Vol. 47, No. SUPPL.4, 08.2012, p. 228-232.

    Research output: Contribution to journalArticle

    Dissen, Gregory ; Lomniczi, Alejandro ; Boudreau, R. L. ; Chen, Y. H. ; Davidson, B. L. ; Ojeda, Sergio. / Targeted gene silencing to induce permanent sterility. In: Reproduction in Domestic Animals. 2012 ; Vol. 47, No. SUPPL.4. pp. 228-232.
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