Tagmentation-based library preparation for low DNA input whole genome bisulfite sequencing

Dieter Weichenhan, Qi Wang, Andrew Adey, Stephan Wolf, Jay Shendure, Roland Eils, Christoph Plass

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Aberrations of the DNA methylome contribute to onset and progression of diseases. Whole genome bisulfite sequencing (WGBS) is the only analytical method covering the complete methylome. Alternative methods requiring less DNA than WGBS analyze only a minor portion of the methylome and do not cover important regulatory features like enhancers and noncoding RNAs. In tagmentation-based WGBS (TWGBS), several DNA and time-consuming steps of the conventional WGBS library preparation are circumvented by the use of a hyperactive transposase, which simultaneously fragments DNA and appends sequencing adapters. TWGBS requires only nanogram amounts of DNA and, thus, is well suited to study precious biological specimens such as sorted cells or micro-dissected tissue samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages105-122
Number of pages18
Volume1708
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1708
ISSN (Print)1064-3745

Keywords

  • 5-Methyl cytosine
  • Methylome
  • Tagmentation
  • Transposase
  • Whole genome bisulfite sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Weichenhan, D., Wang, Q., Adey, A., Wolf, S., Shendure, J., Eils, R., & Plass, C. (2018). Tagmentation-based library preparation for low DNA input whole genome bisulfite sequencing. In Methods in Molecular Biology (Vol. 1708, pp. 105-122). (Methods in Molecular Biology; Vol. 1708). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7481-8_6