T lymphocytes of allophenic mice do not reject spleen colony-forming units of the parental genotypes

Lev Fedorov, E. I. Panteleev

Research output: Contribution to journalArticle

Abstract

Self-tolerance in BALB/c mutually implies C57BL/6 and BALB/c mutually implies B10.SM (SM) allophenic mice is examined in this study. Chimerism was determined by coat mosaicism, using electrophoresis of allozyme variants at the glucose phosphate isomerase-1 (GPI-1) in peripheral blood erythrocytes and a cytotoxity test for lymph node (LN) lymphocytes and blood nucleated cells. An age-dependent increase in the percent content of BALB/c erythrocytes was observed in both types of chimeras. The immunological status of chimeras was assessed by T cell interactions with spleen colony-forming cells (SFU-S) able to form colonies in the recipient spleens. LN lymphocytes of the chimeras, together with bone marrow cells (BMC) of the parental genotype, were transplanted to lethally irradiated F1 hybrids. Chimera T lymphocytes did not inactivate CFU-S of the parental genotype and colonies formed in the spleens of F1 hybrids. The absence of splenic colonies in (C x B6)F1 hybrids, in which BMC from B6 had been transplanted (either in combination with chimera lymphocytes or alone), was due to the reaction of host natural killer (NK) cells. The results obtained testify to the permanent immunological tolerance of chimera T lymphocytes to BMC antigens of the both parental genotypes. The chimeric shift in the erythrocyte population is not coupled to disturbances in self-tolerance of chimeric mice.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalJournal of Hematotherapy and Stem Cell Research
Volume9
Issue number4
DOIs
StatePublished - 2000
Externally publishedYes

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Stem Cells
Spleen
Genotype
T-Lymphocytes
Bone Marrow Cells
Self Tolerance
Erythrocytes
Lymphocytes
xylose isomerase
Lymph Nodes
Chimerism
Mosaicism
Cell Communication
Natural Killer Cells
Isoenzymes
Electrophoresis
Blood Cells
Phosphates
Antigens
Population

ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

T lymphocytes of allophenic mice do not reject spleen colony-forming units of the parental genotypes. / Fedorov, Lev; Panteleev, E. I.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 9, No. 4, 2000, p. 517-523.

Research output: Contribution to journalArticle

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