T cell-dependent induction of NF-κB in B cells

Anne Christine Lalmanach-Girard, Thomas C. Chiles, David C. Parker, Thomas L. Rothstein

Research output: Contribution to journalArticlepeer-review

97 Scopus citations


In comparison to B cell stimulation mediated by surface immunoglobulin (Ig) antigen receptor ligation, little is known about the intracellular events associated with T cell-dependent B cell responses. A model for the efferent phase of T cell-B cell interaction was used to examine the capacity of activated T cells to trigger nuclear expression of the trans-acting transcription factor, NF-κB, in B cells. Fixed, activated, but not fixed, resting Th2 cells were found to induce increased binding activity for a κB site-containing oligonucleotide in a time-dependent manner. This induction of NF-κB was eliminated by an antibody directed against a 39-kD cell interaction protein on activated T cells as well as by a soluble form of B cell CD40. Of particular relevance to intracellular signaling, NF-κB induction was not diminished by prior depletion of B cell protein kinase C (PKC) with phorbol myristate acetate. These results strongly suggest that T cell-dependent B cell stimulation is associated with NF-κB induction via p39-CD40 interaction and that this is brought about by non-PKC dependent signaling, in marked contrast to the previously documented requirement for PKC in sIg receptor-mediated stimulation. This suggests that NF-κB responds to more than one receptor-mediated intracellular signaling pathway in B cells and may be part of a “final common pathway” for B cell stimulation.

Original languageEnglish (US)
Pages (from-to)1215-1219
Number of pages5
JournalJournal of Experimental Medicine
Issue number4
StatePublished - Apr 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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