Abstract
In comparison to B cell stimulation mediated by surface immunoglobulin (Ig) antigen receptor ligation, little is known about the intracellular events associated with T cell-dependent B cell responses. A model for the efferent phase of T cell-B cell interaction was used to examine the capacity of activated T cells to trigger nuclear expression of the tirans-acting transcription factor, NF-κB, in B cells. Fixed, activated, but not fixed, resting Th2 cells were found to induce increased binding activity for a κB site-containing oligonucleotide in a time-dependent manner. This induction of NF-κB was eliminated by an antibody directed against a 39-kD cell interaction protein on activated T cells as well as by a soluble form of B cell CD40. Of particular relevance to intracellular signaling, NF-κB induction was not diminished by prior depletion of B cell protein kinase C (PKC) with phorbol myristate acetate. These results strongly suggest that T cell-dependent B cell stimulation is associated with NF-κB induction via p39-CD40 interaction and that this is brought about by non-PKC dependent signaling, in marked contrast to the previously documented requirement for PKC in sIg receptor-mediated stimulation. This suggests that NF-κK responds to more than one receptor-mediated intracellular signaling pathway in B cells and may be part of a "final common pathway" for B cell stimulation.
Original language | English (US) |
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Pages (from-to) | 1215-1219 |
Number of pages | 5 |
Journal | Journal of Experimental Medicine |
Volume | 177 |
Issue number | 4 |
State | Published - Apr 1 1993 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology