T cell-dependent induction of NF-κB in B cells

Anne Christine Lalmanach-Girard, Thomas C. Chiles, David Parker, Thomas L. Rothstein

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

In comparison to B cell stimulation mediated by surface immunoglobulin (Ig) antigen receptor ligation, little is known about the intracellular events associated with T cell-dependent B cell responses. A model for the efferent phase of T cell-B cell interaction was used to examine the capacity of activated T cells to trigger nuclear expression of the tirans-acting transcription factor, NF-κB, in B cells. Fixed, activated, but not fixed, resting Th2 cells were found to induce increased binding activity for a κB site-containing oligonucleotide in a time-dependent manner. This induction of NF-κB was eliminated by an antibody directed against a 39-kD cell interaction protein on activated T cells as well as by a soluble form of B cell CD40. Of particular relevance to intracellular signaling, NF-κB induction was not diminished by prior depletion of B cell protein kinase C (PKC) with phorbol myristate acetate. These results strongly suggest that T cell-dependent B cell stimulation is associated with NF-κB induction via p39-CD40 interaction and that this is brought about by non-PKC dependent signaling, in marked contrast to the previously documented requirement for PKC in sIg receptor-mediated stimulation. This suggests that NF-κK responds to more than one receptor-mediated intracellular signaling pathway in B cells and may be part of a "final common pathway" for B cell stimulation.

Original languageEnglish (US)
Pages (from-to)1215-1219
Number of pages5
JournalJournal of Experimental Medicine
Volume177
Issue number4
StatePublished - Apr 1 1993
Externally publishedYes

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B-Lymphocytes
T-Lymphocytes
Cell Communication
Protein Kinase C
Th2 Cells
B-Cell Antigen Receptors
Antigen Receptors
Tetradecanoylphorbol Acetate
Surface Antigens
Oligonucleotides
Ligation
Transcription Factors
Phosphotransferases
Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Lalmanach-Girard, A. C., Chiles, T. C., Parker, D., & Rothstein, T. L. (1993). T cell-dependent induction of NF-κB in B cells. Journal of Experimental Medicine, 177(4), 1215-1219.

T cell-dependent induction of NF-κB in B cells. / Lalmanach-Girard, Anne Christine; Chiles, Thomas C.; Parker, David; Rothstein, Thomas L.

In: Journal of Experimental Medicine, Vol. 177, No. 4, 01.04.1993, p. 1215-1219.

Research output: Contribution to journalArticle

Lalmanach-Girard, AC, Chiles, TC, Parker, D & Rothstein, TL 1993, 'T cell-dependent induction of NF-κB in B cells', Journal of Experimental Medicine, vol. 177, no. 4, pp. 1215-1219.
Lalmanach-Girard AC, Chiles TC, Parker D, Rothstein TL. T cell-dependent induction of NF-κB in B cells. Journal of Experimental Medicine. 1993 Apr 1;177(4):1215-1219.
Lalmanach-Girard, Anne Christine ; Chiles, Thomas C. ; Parker, David ; Rothstein, Thomas L. / T cell-dependent induction of NF-κB in B cells. In: Journal of Experimental Medicine. 1993 ; Vol. 177, No. 4. pp. 1215-1219.
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