T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration

Mi Ryoung Song, Ryuichi Shirasaki, Chen Leng Cai, Esmeralda C. Ruiz, Sylvia M. Evans, Soo-Kyung Lee, Samuel L. Pfaff

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Members of the T-box transcription factor family (Tbx) are associated with several human syndromes during embryogenesis. Nevertheless, their functions within the developing CNS remain poorly characterized. Tbx20 is expressed by migrating branchiomotor/visceromotor (BM/VM) neurons within the hindbrain during neuronal circuit formation. We examined Tbx20 function in BM/VM cells using conditional Tbx20-null mutant mice to delete the gene in neurons. Hindbrain rhombomere patterning and the initial generation of post-mitotic BM/VM neurons were normal in Tbx20 mutants. However, Tbx20 was required for the tangential (caudal) migration of facial neurons, the lateral migration of trigeminal cells and the trans-median movement of vestibuloacoustic neurons. Facial cell soma migration defects were associated with the coordinate downregulation of multiple components of the planar cell polarity pathway including Fzd7, Wnt11, Prickle1, Vang1 and Vang2. Our study suggests that Tbx20 programs a variety of hindbrain motor neurons for migration, independent of directionality, and in facial neurons is a positive regulator of the non-canonical Wnt signaling pathway.

Original languageEnglish (US)
Pages (from-to)4945-4955
Number of pages11
JournalDevelopment
Volume133
Issue number24
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Motor Neurons
Cell Movement
Transcription Factors
Neurons
Rhombencephalon
Cell Polarity
Wnt Signaling Pathway
Carisoprodol
Embryonic Development
Cell Body
Down-Regulation
Genes

Keywords

  • Branchiomotor
  • Hindbrain
  • Migration
  • Mouse
  • Planar cell polarity
  • Tbx20
  • Wnt

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Song, M. R., Shirasaki, R., Cai, C. L., Ruiz, E. C., Evans, S. M., Lee, S-K., & Pfaff, S. L. (2006). T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. Development, 133(24), 4945-4955. https://doi.org/10.1242/dev.02694

T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. / Song, Mi Ryoung; Shirasaki, Ryuichi; Cai, Chen Leng; Ruiz, Esmeralda C.; Evans, Sylvia M.; Lee, Soo-Kyung; Pfaff, Samuel L.

In: Development, Vol. 133, No. 24, 12.2006, p. 4945-4955.

Research output: Contribution to journalArticle

Song, MR, Shirasaki, R, Cai, CL, Ruiz, EC, Evans, SM, Lee, S-K & Pfaff, SL 2006, 'T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration', Development, vol. 133, no. 24, pp. 4945-4955. https://doi.org/10.1242/dev.02694
Song, Mi Ryoung ; Shirasaki, Ryuichi ; Cai, Chen Leng ; Ruiz, Esmeralda C. ; Evans, Sylvia M. ; Lee, Soo-Kyung ; Pfaff, Samuel L. / T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. In: Development. 2006 ; Vol. 133, No. 24. pp. 4945-4955.
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