Synthetic phosphopeptide immunogens yield activation-specific antibodies to the c-erbB-2 receptor

R. J. Epstein, B. J. Druker, T. M. Roberts, C. D. Stiles

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

We inoculated rabbits with synthetic phosphopeptides, duplicating a major autophosphorylation site of the c-erbB-2 protooncogene product. The rabbits produced antisera that, after reverse immunoaffinity purification, selectively recognize the erbB-2 protein in its enzymatically active configuration. These anti-phosphopeptide antisera identify a subset of erbB- 2-positive human cell lines wherein the protein is constitutively active as a tyrosine kinase. Synthetic phosphopeptides incorporating informative protein phosphorylation sites may prove useful for generating antibodies that indicate the activation state of additional tyrosine kinases and perhaps other proteins phosphorylated on serine and threonine residues.

Original languageEnglish (US)
Pages (from-to)10435-10439
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
DOIs
StatePublished - Nov 17 1992
Externally publishedYes

Keywords

  • breast neoplasms
  • oncogenes
  • phosphorylation
  • tyrosine kinases

ASJC Scopus subject areas

  • General

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