TY - JOUR
T1 - Synthesis of erythrocyte-specific proteins in cultured friend leukemia cells
AU - Kabat, David
AU - Sherton, Corinne C.
AU - Evans, Leonard H.
AU - Bigley, Robert
AU - Koler, Robert D.
N1 - Funding Information:
This work was supported by USPHS grants. by a post-doctoral fellowship from the NCI.
PY - 1975/7
Y1 - 1975/7
N2 - We have studied synthesis of specific proteins in two permanent ilness of Friend virus-induced erythroleukemia cells (Friend line 745 and Ostertag line FSD-1, both derived from DBA/2 mice). By 96 hr following treatment with 1-2% dimethyl sulfoxide (Me2SO), up to 25% of the protein being synthesized by both these cultures is hemoglobin. At that time, hemoglobin constitutes up to 10% of the cellular soluble protein. Both lines synthesize heme and globin coordinately, and α and β globin chains in a nearly balanced 1:1 ratio. However, the ratio of βMajor:βMinor chains synthesized by these induced Friend leukemia (FL) cells is approximately 9 in the FSD-1 line and 1.3 in the Friend Clone 745 line, whereas it is 4 in normal adult DBA/2 mouse erythrocytes. Evidence for the latter conclusion was obtained by electrophoresis of FL hemoglobins on cellulose acetate membranes, and also by chromatographic separation of α, βMajor, and βMinor globins on carboxymethylcellulose in 8 M urea at 20°C. Carbonic anhydrase activity per mg protein is 3 times higher in induced than in control cultures. 2,3-diphosphoglyceric acid is not found in induced FL cells. Induced and control FL cells agglutinate strongly and equally with Phaseolus vulgaris phytohemagglutinin. The developmental process in these cultured leukemia cells appears to be an aberrant erythropolesis.
AB - We have studied synthesis of specific proteins in two permanent ilness of Friend virus-induced erythroleukemia cells (Friend line 745 and Ostertag line FSD-1, both derived from DBA/2 mice). By 96 hr following treatment with 1-2% dimethyl sulfoxide (Me2SO), up to 25% of the protein being synthesized by both these cultures is hemoglobin. At that time, hemoglobin constitutes up to 10% of the cellular soluble protein. Both lines synthesize heme and globin coordinately, and α and β globin chains in a nearly balanced 1:1 ratio. However, the ratio of βMajor:βMinor chains synthesized by these induced Friend leukemia (FL) cells is approximately 9 in the FSD-1 line and 1.3 in the Friend Clone 745 line, whereas it is 4 in normal adult DBA/2 mouse erythrocytes. Evidence for the latter conclusion was obtained by electrophoresis of FL hemoglobins on cellulose acetate membranes, and also by chromatographic separation of α, βMajor, and βMinor globins on carboxymethylcellulose in 8 M urea at 20°C. Carbonic anhydrase activity per mg protein is 3 times higher in induced than in control cultures. 2,3-diphosphoglyceric acid is not found in induced FL cells. Induced and control FL cells agglutinate strongly and equally with Phaseolus vulgaris phytohemagglutinin. The developmental process in these cultured leukemia cells appears to be an aberrant erythropolesis.
UR - http://www.scopus.com/inward/record.url?scp=0016814715&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016814715&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(75)90109-9
DO - 10.1016/0092-8674(75)90109-9
M3 - Article
C2 - 807334
AN - SCOPUS:0016814715
SN - 0092-8674
VL - 5
SP - 331
EP - 338
JO - Cell
JF - Cell
IS - 3
ER -