Synthesis of basic fibroblast growth factor by murine mast cells. Regulation by transforming growth factor beta, tumor necrosis factor alpha, and stem cell factor

Zhenhong Qu, Xiaona Huang, Proochista Ahmadi, Paula Stenberg, Janice M. Liebler, Anh Chi Le, Stephen Planck, James (Jim) Rosenbaum

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Abstract

Background: Mast cells (MC) are involved in a wide spectrum of disorders characterized by neovascularization and fibroproliferation. We and others recently reported that human MC are a source of basic fibroblast growth factor (b FGF-2), a potent angiogenic and mitogenic polypeptide, in several disease conditions, such as chronic inflammation, hemangioma, and benign cutaneous mastocytosis. These findings suggest that FGF-2 may be an important mediator of cell proliferation and angiogenesis associated with MC. Since MC are heterogeneous across species, it is unknown whether FGF-2 expression is a feature common to all MC, or whether FGF-2 expression by MC can be regulated. We therefore examined FGF-2 expression by MC in mouse tissue and MC lines. Methods: Immunostaining, RT-PCR, ELISA, immunoblot and Northern blot analyses were employed to study four murine MC lines for FGF-2 expression and its regulation by transforming growth factor-β (TGF-β), stem cell factor (SCF), and tumor necrosis factor-α (TNF-α). Results: Mouse tissue MC and three of four murine MC lines (CFTL-12, CFTL-15, ABFTL-3) express FGF-2 as judged by immunostaining, ELISA, Western blot and Northern blot analyses, and reverse transcription-polymerase chain reaction. While TNF-α appeared to downregulate FGF-2 mRNA levels, treatment with SCF or TGF-β resulted in an increase in the expression of FGF-2 at mRNA level which can be attenuated by TNF-α. However, the concurrent increase in FGF-2 protein was negligible, possibly due to immaturity of these cell lines. Conclusion: Expression of FGF-2 may be a ubiquitous feature of MC in other species in addition to humans, and can be selectively regulated by SCF, TGF-β and TNF-α.

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Keywords

  • Fibroblast growth factor
  • Mast cell
  • Stem cell factor
  • Transforming growth factor
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Synthesis of basic fibroblast growth factor by murine mast cells. Regulation by transforming growth factor beta, tumor necrosis factor alpha, and stem cell factor. / Qu, Zhenhong; Huang, Xiaona; Ahmadi, Proochista; Stenberg, Paula; Liebler, Janice M.; Le, Anh Chi; Planck, Stephen; Rosenbaum, James (Jim).

In: International Archives of Allergy and Immunology, Vol. 115, No. 1, 01.1998, p. 47-54.

Research output: Contribution to journalArticle