Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

Samantha R. Levine, Kimberly Beatty

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1′-cyclohexane]-2′,5′-diene-2,4′-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as "turn-on" substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.

Original languageEnglish (US)
Pages (from-to)1835-1838
Number of pages4
JournalChemical Communications
Volume52
Issue number9
DOIs
StatePublished - Jan 31 2016

Fingerprint

Fluorophores
Esterases
Cells
Acridines
Lipases
Cyclohexane
Lipase
Fluorescence
Substrates
methylamphotericin B

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

Cite this

Synthesis of a far-red fluorophore and its use as an esterase probe in living cells. / Levine, Samantha R.; Beatty, Kimberly.

In: Chemical Communications, Vol. 52, No. 9, 31.01.2016, p. 1835-1838.

Research output: Contribution to journalArticle

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