Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

Samantha R. Levine, Kimberly E. Beatty

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1′-cyclohexane]-2′,5′-diene-2,4′-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as "turn-on" substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.

Original languageEnglish (US)
Pages (from-to)1835-1838
Number of pages4
JournalChemical Communications
Volume52
Issue number9
DOIs
StatePublished - Jan 31 2016

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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