Synthesis and characterization of a spin‐labeled aminoacyl transfer ribonucleic acid

David Kabat, Brian Hoffman, Alexander Rich

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Unfractionated yeast transfer ribonucleic acid (tRNA) was reacted in aqueous acetone solution with the sulfhydryl spin‐labeling reagnent, N‐(l‐oxyl‐2,2,5,5‐tetramethyl‐3‐Pyrrolidinyl)iodoacetamide. Whereas tRNA stripped of amino acids reacted only slowly, there were sites on tRNA charged with cysteine which combined rapidly with the reagent. The latter class of spin‐label was quickly cleaved from the tRNA upon incubation in mildly alkaline solution (pH 8.0), suggesting that it was attached to the cysteinyl side chains. The paramagnetic resonance spectrum of column‐purified spin‐labeled cysteinyl tRNA showed that the spin‐label was partially immobilized as a result of its interaction with the tRNA. When the tRNA was slowly heated, an abrupt increase occurred in the rotational mobility of the paramagnetic amino‐acid side chain.

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalBiopolymers
Volume9
Issue number1
DOIs
StatePublished - 1970

Fingerprint

Transfer RNA
Iodoacetamide
Electron Spin Resonance Spectroscopy
Acetone
Yeast
Cysteine
Paramagnetic resonance
Amino acids
Yeasts
Amino Acids

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Cite this

Synthesis and characterization of a spin‐labeled aminoacyl transfer ribonucleic acid. / Kabat, David; Hoffman, Brian; Rich, Alexander.

In: Biopolymers, Vol. 9, No. 1, 1970, p. 95-101.

Research output: Contribution to journalArticle

Kabat, David ; Hoffman, Brian ; Rich, Alexander. / Synthesis and characterization of a spin‐labeled aminoacyl transfer ribonucleic acid. In: Biopolymers. 1970 ; Vol. 9, No. 1. pp. 95-101.
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