Abstract
Unfractionated yeast transfer ribonucleic acid (tRNA) was reacted in aqueous acetone solution with the sulfhydryl spin‐labeling reagnent, N‐(l‐oxyl‐2,2,5,5‐tetramethyl‐3‐Pyrrolidinyl)iodoacetamide. Whereas tRNA stripped of amino acids reacted only slowly, there were sites on tRNA charged with cysteine which combined rapidly with the reagent. The latter class of spin‐label was quickly cleaved from the tRNA upon incubation in mildly alkaline solution (pH 8.0), suggesting that it was attached to the cysteinyl side chains. The paramagnetic resonance spectrum of column‐purified spin‐labeled cysteinyl tRNA showed that the spin‐label was partially immobilized as a result of its interaction with the tRNA. When the tRNA was slowly heated, an abrupt increase occurred in the rotational mobility of the paramagnetic amino‐acid side chain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-101 |
| Number of pages | 7 |
| Journal | Biopolymers |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1970 |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Biomaterials
- Organic Chemistry
Cite this
Synthesis and characterization of a spin‐labeled aminoacyl transfer ribonucleic acid. / Kabat, David; Hoffman, Brian; Rich, Alexander.
In: Biopolymers, Vol. 9, No. 1, 1970, p. 95-101.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis and characterization of a spin‐labeled aminoacyl transfer ribonucleic acid
AU - Kabat, David
AU - Hoffman, Brian
AU - Rich, Alexander
PY - 1970
Y1 - 1970
N2 - Unfractionated yeast transfer ribonucleic acid (tRNA) was reacted in aqueous acetone solution with the sulfhydryl spin‐labeling reagnent, N‐(l‐oxyl‐2,2,5,5‐tetramethyl‐3‐Pyrrolidinyl)iodoacetamide. Whereas tRNA stripped of amino acids reacted only slowly, there were sites on tRNA charged with cysteine which combined rapidly with the reagent. The latter class of spin‐label was quickly cleaved from the tRNA upon incubation in mildly alkaline solution (pH 8.0), suggesting that it was attached to the cysteinyl side chains. The paramagnetic resonance spectrum of column‐purified spin‐labeled cysteinyl tRNA showed that the spin‐label was partially immobilized as a result of its interaction with the tRNA. When the tRNA was slowly heated, an abrupt increase occurred in the rotational mobility of the paramagnetic amino‐acid side chain.
AB - Unfractionated yeast transfer ribonucleic acid (tRNA) was reacted in aqueous acetone solution with the sulfhydryl spin‐labeling reagnent, N‐(l‐oxyl‐2,2,5,5‐tetramethyl‐3‐Pyrrolidinyl)iodoacetamide. Whereas tRNA stripped of amino acids reacted only slowly, there were sites on tRNA charged with cysteine which combined rapidly with the reagent. The latter class of spin‐label was quickly cleaved from the tRNA upon incubation in mildly alkaline solution (pH 8.0), suggesting that it was attached to the cysteinyl side chains. The paramagnetic resonance spectrum of column‐purified spin‐labeled cysteinyl tRNA showed that the spin‐label was partially immobilized as a result of its interaction with the tRNA. When the tRNA was slowly heated, an abrupt increase occurred in the rotational mobility of the paramagnetic amino‐acid side chain.
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U2 - 10.1002/bip.1970.360090108
DO - 10.1002/bip.1970.360090108
M3 - Article
VL - 9
SP - 95
EP - 101
JO - Biopolymers - Nucleic Acid Sciences Section
JF - Biopolymers - Nucleic Acid Sciences Section
SN - 0006-3525
IS - 1
ER -