Synthesis and characterization of a recombinant myohemerythrin protein encoded by a synthetic gene

Hannah Alexander, Stephen Alexander, Fred Heffron, Terry M. Fieser, Beverly N. Hay, Elizabeth D. Getzoff, John A. Tainer, Richard A. Lerner

    Research output: Contribution to journalArticle

    6 Scopus citations

    Abstract

    The antigenic epitopes of the myohemerythrin (MHr) molecule have been studied extensively. The critical amino acid residues responsible for its immune recognition have been identified by using synthetic peptides and the technique ofepitope scanning. To assess the true relevance of these techniques for determining the molecular mechanism of antigenic recognition and immunogenicity, the results obtained with isolated peptides should be tested in the context of the folded protein. To this end, we have designed and constructed a synthetic MHr gene, in modular form, which will allow subsequent alterations of nucleotide sequences encoding epitopes of interest. We have produced the recombinant protein at high level, and have shown by several criteria that it possesses the chemical, physical and immunological properties of the native worm protein. Thus, we have developed a valuable system for detailed immunological studies of the structure and chemistry required for antibody binding to protein.

    Original languageEnglish (US)
    Pages (from-to)151-156
    Number of pages6
    JournalGene
    Volume99
    Issue number2
    DOIs
    StatePublished - Mar 15 1991

    Keywords

    • Recombinant DNA
    • critical residues
    • gene construction
    • immune recognition
    • protein antigenicity
    • synthetic oligodeoxyribonucleotide

    ASJC Scopus subject areas

    • Genetics

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  • Cite this

    Alexander, H., Alexander, S., Heffron, F., Fieser, T. M., Hay, B. N., Getzoff, E. D., Tainer, J. A., & Lerner, R. A. (1991). Synthesis and characterization of a recombinant myohemerythrin protein encoded by a synthetic gene. Gene, 99(2), 151-156. https://doi.org/10.1016/0378-1119(91)90121-Q