Syngeneic schwann cell transplantation preserves vision in RCS rat without immunosuppression

Trevor McGill, Raymond D. Lund, Robert M. Douglas, Shaomei Wang, Bin Lu, Byron D. Silver, Matt R. Secretan, Jennifer N. Arthur, Glen T. Prusky

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

PURPOSE. To evaluate the efficacy of immunologically compatible Schwann cells transplanted without immunosuppression in the RCS rat retina to preserve vision. METHODS. Syngeneic (dystrophic RCS) Schwann cells harvested from sciatic nerves were cultured and transplanted into one eye of dystrophic RCS rats at an early stage of retinal degeneration. Allogeneic (Long-Evans) Schwann cells and unoperated eyes served as controls. Vision through transplanted and unoperated eyes was then quantified using two visual behavior tasks, one measuring the spatial frequency and contrast sensitivity thresholds of the optokinetic response (OKR) and the other measuring grating acuity in a perception task. RESULTS. Spatial frequency thresholds measured through syngeneically transplanted eyes maintained near normal spatial frequency sensitivity for approximately 30 weeks, whereas thresholds through control eyes deteriorated to less than 20% of normal over the same period. Contrast sensitivity was preserved through syngeneically transplanted eyes better than through allogeneic and unoperated eyes, at all spatial frequencies. Grating acuity measured through syngeneically transplanted eyes was maintained at approximately 60% of normal, whereas acuity of allogeneically transplanted eyes was significantly lower at approximately 40% of normal. CONCLUSIONS. The ability of immunoprivileged Schwann cell transplants to preserve vision in RCS rats indicates that transplantation of syngeneic Schwann cells holds promise as a preventive treatment for retinal degenerative disease.

Original languageEnglish (US)
Pages (from-to)1906-1912
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume48
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

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Schwann Cells
Cell Transplantation
Immunosuppression
Contrast Sensitivity
Isogeneic Transplantation
Retinal Diseases
Retinal Degeneration
Aptitude
Sciatic Nerve
Retina
Transplants

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Syngeneic schwann cell transplantation preserves vision in RCS rat without immunosuppression. / McGill, Trevor; Lund, Raymond D.; Douglas, Robert M.; Wang, Shaomei; Lu, Bin; Silver, Byron D.; Secretan, Matt R.; Arthur, Jennifer N.; Prusky, Glen T.

In: Investigative Ophthalmology and Visual Science, Vol. 48, No. 4, 04.2007, p. 1906-1912.

Research output: Contribution to journalArticle

McGill, T, Lund, RD, Douglas, RM, Wang, S, Lu, B, Silver, BD, Secretan, MR, Arthur, JN & Prusky, GT 2007, 'Syngeneic schwann cell transplantation preserves vision in RCS rat without immunosuppression', Investigative Ophthalmology and Visual Science, vol. 48, no. 4, pp. 1906-1912. https://doi.org/10.1167/iovs.06-1117
McGill, Trevor ; Lund, Raymond D. ; Douglas, Robert M. ; Wang, Shaomei ; Lu, Bin ; Silver, Byron D. ; Secretan, Matt R. ; Arthur, Jennifer N. ; Prusky, Glen T. / Syngeneic schwann cell transplantation preserves vision in RCS rat without immunosuppression. In: Investigative Ophthalmology and Visual Science. 2007 ; Vol. 48, No. 4. pp. 1906-1912.
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