Synergism between rhinovirus infection and oxidant pollutant exposure ehances airway epithelial cell cytokine production

E. William Spannhake, Sekhar P M Reddy, David Jacoby, Xiao Ying Yu, Bahman Saatian, Jingyan Tian

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Of the several factors believed to exacerbate asthmatic symptoms, air pollution and viral infections are considered to be particularly important. Although evidence indicates that each of these respiratory insults individually can increase asthma severity in susceptible individuals, we know little about the extent to which exposure to environmental oxidant pollutants can influence the course of respiratory viral infection and its associated inflammation. To investigate the interaction of these two stimuli within their common epithelial cell targets in the upper and lower respiratory tracks, we infected primary human nasal epithelial cells and cells of the BEAS-2B line grown at the air-liquid interface with human rhinovirus type 16 (RV16) and exposed them to NO2 (2.0 ppm) or O3 (0.2 ppm) for 3 hr. Independently, RV16, NO2, and O3 rapidly increased release of the inflammatory cytokine interleukin-8 through oxidant-dependent mechanisms. The combined effect of RV16 and oxidant ranged from 42% to 250% greater than additive for NO2 and from 41% to 67% for O3. We abrogated these effects by treating the cells with the antioxidant N-acetylcysteine. Surface expression of intercellular adhesion molecule 1 (ICAM-1) underwent additive enhancement in response to combined stimulation. These data indicate that oxidant pollutants can amplify the generation of proinflammatory cytokines by RV16-infected cells and suggest that virus-induced inflammation in upper and lower airways may be exacerbated by concurrent exposure to ambient levels of oxidants commonly encountered the indoor and outdoor environments.

Original languageEnglish (US)
Pages (from-to)665-670
Number of pages6
JournalEnvironmental Health Perspectives
Volume110
Issue number7
StatePublished - 2002
Externally publishedYes

Fingerprint

Rhinovirus
synergism
Oxidants
oxidant
Epithelial Cells
Cytokines
Infection
Virus Diseases
Inflammation
Environmental Pollutants
pollutant
asthma
Air Pollution
Acetylcysteine
Intercellular Adhesion Molecule-1
Air pollution
adhesion
Interleukin-8
Viruses
Nose

Keywords

  • Bronchial epithelium
  • ICAM-1
  • IL-8
  • Nasal epithelium
  • Nitrogen dioxide
  • Oxidant stress
  • Ozone

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Synergism between rhinovirus infection and oxidant pollutant exposure ehances airway epithelial cell cytokine production. / William Spannhake, E.; Reddy, Sekhar P M; Jacoby, David; Yu, Xiao Ying; Saatian, Bahman; Tian, Jingyan.

In: Environmental Health Perspectives, Vol. 110, No. 7, 2002, p. 665-670.

Research output: Contribution to journalArticle

William Spannhake, E. ; Reddy, Sekhar P M ; Jacoby, David ; Yu, Xiao Ying ; Saatian, Bahman ; Tian, Jingyan. / Synergism between rhinovirus infection and oxidant pollutant exposure ehances airway epithelial cell cytokine production. In: Environmental Health Perspectives. 2002 ; Vol. 110, No. 7. pp. 665-670.
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