Synaptic and extrasynaptic NMDA receptor NR2 subunits in cultured hippocampal neurons

Christopher G. Thomas, Ashleigh J. Miller, Gary Westbrook

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Early in development, neurons only express NR1/NR2B-containing N-methyl-D-aspartate (NMDA) receptors. Later, NR2A subunits are upregulated during a period of rapid synapse formation. This pattern is often interpreted to indicate that NR2A-containing receptors are synaptic and that NR2B-containing receptors are extrasynaptic. We re-examined this issue using whole cell recordings in cultured hippocampal neurons. As expected, the inhibition of whole cell currents by the NR2B-specific antagonist, ifenprodil, progressively decreased from 69.5 ± 2.4% [6 days in vitro (DIV)] to 54.9 ± 2.6% (8 DIV), before reaching a plateau in the second week (42.5 ± 2%, 12-19 DIV). In NR2A-/- neurons, which express only NR1/NR2B-containing NMDA receptors, autaptic excitatory postsynaptic currents (EPSCs; ≥12 DIV) were more sensitive to ifenprodil and decayed more slowly than EPSCs in wild-type neurons. Thus NR2B-containing receptors were not excluded from synapses. We blocked synaptic NMDA receptors with MK-801 during evoked transmitter release, thus allowing us to isolate extrasynaptic receptors. Ifenprodil inhibition of this extrasynaptic population was highly variable in different neurons. Furthermore, extrasynaptic receptors in autaptic cultures were only partially blocked by ifenprodil, indicating that NR2A-containing receptors are not exclusively confined to the synapse. Extrasynaptic NR2A-containing receptors were also detected in NR2A-/- neurons transfected with full-length NR2A. Truncation of the NR2A C terminus did not eliminate synaptic expression of NR2A-containing receptors. Our results indicate that NR2A- and NR2B-containing receptors can be located in either synaptic or extrasynaptic compartments.

Original languageEnglish (US)
Pages (from-to)1727-1734
Number of pages8
JournalJournal of Neurophysiology
Volume95
Issue number3
DOIs
StatePublished - Mar 2006

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N-Methyl-D-Aspartate Receptors
Neurons
Synapses
Neurotransmitter Receptor
Dizocilpine Maleate
Excitatory Postsynaptic Potentials
Patch-Clamp Techniques
ifenprodil
In Vitro Techniques
Population

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Synaptic and extrasynaptic NMDA receptor NR2 subunits in cultured hippocampal neurons. / Thomas, Christopher G.; Miller, Ashleigh J.; Westbrook, Gary.

In: Journal of Neurophysiology, Vol. 95, No. 3, 03.2006, p. 1727-1734.

Research output: Contribution to journalArticle

Thomas, Christopher G. ; Miller, Ashleigh J. ; Westbrook, Gary. / Synaptic and extrasynaptic NMDA receptor NR2 subunits in cultured hippocampal neurons. In: Journal of Neurophysiology. 2006 ; Vol. 95, No. 3. pp. 1727-1734.
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