SWI/SNF chromatin-remodeling factors induce changes in DNA methylation to promote transcriptional activation

Fatima Banine, Christopher Bartlett, Ranjaka Gunawardena, Christian Muchardt, Moshe Yaniv, Erik S. Knudsen, Bernard E. Weissman, Lawrence (Larry) Sherman

    Research output: Contribution to journalArticle

    82 Citations (Scopus)

    Abstract

    Brahma (Brm) and brahma-related gene-1 (Brg1) are mammalian homologues of SWI/SNF chromatin-remodeling factor subunits that can regulate both transcriptional activation and repression. Both Brg1 and Brm are mutated or deleted in numerous cancer cell lines, leading to the altered expression of genes that influence cell proliferation and metastasis. Here, we find that the promoters of two such genes, CD44 and E-cadherin, are hypermethylated in cells that have lost Brg1 or Brm. In two carcinoma cell lines that lack functional Brg1 and Brm, CD44 and E-cadherin expression are induced by the demethylating agent 5-aza-2′-deoxycytidine. Transfection with either Brg1 or Brm also induces CD44 and E-cadherin transcription and protein expression in these cells, as well as loss of methylation at sequences in the promoters of both genes. Chromatin immunoprecipitation assays show that Brg1 and Brm associate with these regions of the CD44 and E-cadherin promoters, suggesting that SWI/SNF protein complexes may directly influence the loss of DNA methylation. In vivo, Brm-deficient mice also show methylation and silencing of the CD44 promoter. Collectively, these data implicate loss of SWI/SNF-mediated transcriptional activation as a novel mechanism to increase DNA methylation in cancer cells and provide insight into the mechanisms underlying aberrant gene induction and repression during tumor progression.

    Original languageEnglish (US)
    Pages (from-to)3542-3547
    Number of pages6
    JournalCancer Research
    Volume65
    Issue number9
    DOIs
    StatePublished - May 1 2005

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    Chromatin Assembly and Disassembly
    DNA Methylation
    Transcriptional Activation
    Cadherins
    Genes
    decitabine
    Methylation
    Cell Line
    Neoplasms
    Chromatin Immunoprecipitation
    Transfection
    Proteins
    Cell Proliferation
    Neoplasm Metastasis
    Carcinoma
    Gene Expression

    ASJC Scopus subject areas

    • Cancer Research
    • Oncology

    Cite this

    SWI/SNF chromatin-remodeling factors induce changes in DNA methylation to promote transcriptional activation. / Banine, Fatima; Bartlett, Christopher; Gunawardena, Ranjaka; Muchardt, Christian; Yaniv, Moshe; Knudsen, Erik S.; Weissman, Bernard E.; Sherman, Lawrence (Larry).

    In: Cancer Research, Vol. 65, No. 9, 01.05.2005, p. 3542-3547.

    Research output: Contribution to journalArticle

    Banine, Fatima ; Bartlett, Christopher ; Gunawardena, Ranjaka ; Muchardt, Christian ; Yaniv, Moshe ; Knudsen, Erik S. ; Weissman, Bernard E. ; Sherman, Lawrence (Larry). / SWI/SNF chromatin-remodeling factors induce changes in DNA methylation to promote transcriptional activation. In: Cancer Research. 2005 ; Vol. 65, No. 9. pp. 3542-3547.
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