Sustaining sleep spindles through enhanced SK2-channel activity consolidates sleep and elevates arousal threshold

Ralf D. Wimmer, Simone Astori, Chris T. Bond, Zita Rovó, Jean Yves Chatton, John Adelman, Paul Franken, Anita Lüthi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Sleep spindles are synchronized 11-15 Hz electroencephalographic (EEG) oscillations predominant during nonrapid-eye-movement sleep (NREMS). Rhythmic bursting in the reticular thalamic nucleus (nRt), arising from interplay between Cav3.3-type Ca 2+ channels and Ca 2+-dependent small-conductance-type 2 (SK2) K+ channels, underlies spindle generation. Correlative evidence indicates that spindles contribute to memory consolidation and protection against environmental noise in human NREMS. Here, we describe a molecular mechanism through which spindle power is selectively extended and we probed the actions of intensified spindling in the naturally sleeping mouse. Using electrophysiological recordings in acute brain slices from SK2 channel-overexpressing (SK2-OE) mice, we found that nRt bursting was potentiated and thalamic circuit oscillations were prolonged. Moreover, nRt cells showed greater resilience to transit from burst to tonic discharge in response to gradual depolarization, mimicking transitions out of NREMS. Compared with wild-type littermates, chronic EEG recordings of SK2-OE mice contained less fragmented NREMS, while the NREMS EEG power spectrum was conserved. Furthermore, EEG spindle activity was prolonged at NREMS exit. Finally, when exposed to white noise, SK2-OE mice needed stronger stimuli to arouse. Increased nRt bursting thus strengthens spindles and improves sleep quality through mechanisms independent of EEG slow waves (

Original languageEnglish (US)
Pages (from-to)13917-13928
Number of pages12
JournalJournal of Neuroscience
Volume32
Issue number40
DOIs
StatePublished - Oct 3 2012

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Arousal
Sleep
Eye Movements
Thalamic Nuclei
Conservation of Natural Resources
Noise
Brain
Mouse Kcnn2 protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wimmer, R. D., Astori, S., Bond, C. T., Rovó, Z., Chatton, J. Y., Adelman, J., ... Lüthi, A. (2012). Sustaining sleep spindles through enhanced SK2-channel activity consolidates sleep and elevates arousal threshold. Journal of Neuroscience, 32(40), 13917-13928. https://doi.org/10.1523/JNEUROSCI.2313-12.2012

Sustaining sleep spindles through enhanced SK2-channel activity consolidates sleep and elevates arousal threshold. / Wimmer, Ralf D.; Astori, Simone; Bond, Chris T.; Rovó, Zita; Chatton, Jean Yves; Adelman, John; Franken, Paul; Lüthi, Anita.

In: Journal of Neuroscience, Vol. 32, No. 40, 03.10.2012, p. 13917-13928.

Research output: Contribution to journalArticle

Wimmer, RD, Astori, S, Bond, CT, Rovó, Z, Chatton, JY, Adelman, J, Franken, P & Lüthi, A 2012, 'Sustaining sleep spindles through enhanced SK2-channel activity consolidates sleep and elevates arousal threshold', Journal of Neuroscience, vol. 32, no. 40, pp. 13917-13928. https://doi.org/10.1523/JNEUROSCI.2313-12.2012
Wimmer, Ralf D. ; Astori, Simone ; Bond, Chris T. ; Rovó, Zita ; Chatton, Jean Yves ; Adelman, John ; Franken, Paul ; Lüthi, Anita. / Sustaining sleep spindles through enhanced SK2-channel activity consolidates sleep and elevates arousal threshold. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 40. pp. 13917-13928.
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