Sustained phosphorylation of Bid is a marker for resistance to fas-induced apoptosis during chronic liver diseases

Arndt Vogel, Joseph E. Aslan, Holger Willenbring, Christian Klein, Milton Finegold, Howard Mount, Gary Thomas, Markus Grompe

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Background & Aims: Increased rates of apoptosis have been reported to play a role in the pathophysiology of many disorders, including liver diseases. Conversely, genetic mutations that result in impairment of programmed cell death have been associated with cancer development. However, apoptosis resistance can also be the result of nongenetic stress adaptation, as seen in the cancer-prone metabolic liver disease hereditary tyrosinemia. To clarify whether stress-induced apoptosis resistance is a general feature of chronic liver diseases, an animal model of chronic cholestasis was examined. Methods: Studies were performed with mice before and 2 weeks following bile duct ligation and with Fah-/- and Fah/p21-/- mice before and after NTBC withdrawal. Results: Here we show that bile duct ligation induced profound resistance against Fas monoclonal antibody-mediated hepatocyte death. The apoptosis signaling pathway was blocked downstream of caspase-8 activation and proximal to mitochondrial cytochrome c release. In controls, activation of the Fas receptor resulted in rapid dephosphorylation of Bid and its subsequent cleavage, whereas Bid remained phosphorylated and uncleaved in chronic cholestasis and other models of hepatic apoptosis resistance. Conclusions: We propose a model in which the phosphorylation status of Bid determines the apoptotic threshold of hepatocytes in vivo. Furthermore, resistance to apoptosis in chronic cholestasis may contribute to the long-term risk of cancer in this setting.

Original languageEnglish (US)
Pages (from-to)104-119
Number of pages16
JournalGastroenterology
Volume130
Issue number1
DOIs
StatePublished - Jan 2006

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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