Sustained hypertension increases the density of AMPA receptor subunit, GluR1, in baroreceptive regions of the nucleus tractus solitarii of the rat

Sam M. Hermes, Jennifer L. Mitchell, Marc B. Silverman, Patrick J. Lynch, Brenda L. McKee, Timothy W. Bailey, Michael Andresen, Sue Aicher

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

AMPA-type glutamate receptors in the nucleus tractus solitarii (NTS) are necessary for the baroreceptor reflex, a primary mechanism for homeostatic regulation of blood pressure. Within NTS, the GluR1 subunit of the AMPA receptor is found primarily in dendritic spines. We previously showed that both GluR1 and dendritic spine density are increased in NTS of spontaneously hypertensive rats (SHRs). We hypothesize that both receptor and synaptic plasticity are induced by a sustained elevation in arterial pressure. To test the general nature of this hypothesis, we examined whether similar changes in GluR1 density are found in a renovascular model of hypertension, the DOCA-salt rat, and if these changes are preventable by normalizing blood pressure with hydralazine, a peripherally acting vasodilator. Using immunoperoxidase detection, GluR1 appears as small puncta at the light microscopic level, and is found in dendritic spines at the ultrastructural level. Following the development of hypertension, GluR1 spine and puncta counts were significantly greater in DOCA-salt rats than controls. Hydralazine treatment (4-5 weeks) prevented the development of hypertension in DOCA-salt rats and reduced blood pressure of SHRs to normotensive levels. The density of GluR1 puncta in the NTS was significantly reduced by hydralazine treatment in the SHR model. These results show that hypertension alters dendritic spines containing AMPA-type glutamate receptors within NTS, suggesting that adjustments in GluR1 expression within NTS are part of the synaptic adaptations to the hypertensive state.

Original languageEnglish (US)
Pages (from-to)125-136
Number of pages12
JournalBrain Research
Volume1187
Issue number1
DOIs
StatePublished - Jan 2 2008

Fingerprint

Solitary Nucleus
Dendritic Spines
Desoxycorticosterone Acetate
Hypertension
Hydralazine
Inbred SHR Rats
AMPA Receptors
Salts
Glutamate Receptors
Blood Pressure
Renovascular Hypertension
Neuronal Plasticity
Baroreflex
Vasodilator Agents
AMPA 1 glutamate receptor ionotropic
Arterial Pressure
Spine
Light
Therapeutics

Keywords

  • Blood pressure
  • DOCA-salt
  • Electron microscopy
  • Glutamate receptor
  • Immunocytochemistry
  • SHR

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Sustained hypertension increases the density of AMPA receptor subunit, GluR1, in baroreceptive regions of the nucleus tractus solitarii of the rat. / Hermes, Sam M.; Mitchell, Jennifer L.; Silverman, Marc B.; Lynch, Patrick J.; McKee, Brenda L.; Bailey, Timothy W.; Andresen, Michael; Aicher, Sue.

In: Brain Research, Vol. 1187, No. 1, 02.01.2008, p. 125-136.

Research output: Contribution to journalArticle

Hermes, Sam M. ; Mitchell, Jennifer L. ; Silverman, Marc B. ; Lynch, Patrick J. ; McKee, Brenda L. ; Bailey, Timothy W. ; Andresen, Michael ; Aicher, Sue. / Sustained hypertension increases the density of AMPA receptor subunit, GluR1, in baroreceptive regions of the nucleus tractus solitarii of the rat. In: Brain Research. 2008 ; Vol. 1187, No. 1. pp. 125-136.
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