Susceptibility to endotoxin induced uveitis is not reduced in mice deficient in BLT1, the high affinity leukotriene B4 receptor

J. R. Smith; K. Subbarao; D. T. Franc; B. Haribabu; J. T. Rosenbaum

doi:10.1136/bjo.2003.027243

Susceptibility to endotoxin induced uveitis is not reduced in mice deficient in BLT1, the high affinity leukotriene B₄ receptor

J. R. Smith, K. Subbarao, D. T. Franc, B. Haribabu, J. T. Rosenbaum

Ophthalmology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Aim: To investigate the role of arachidonic acid derived chemotactic factor, LTB₄, in the development of endotoxin induced uveitis (EIU), using mice deficient in the BLT1 gene which encodes the high affinity LTB4 receptor. Methods: BLT1 gene deficient and wild type BALB/c mice were injected intravitreally with Escherichia coli 055:B5 lipopolysaccharide (250 ng/2 μl). Number of leukocytes invading the anterior chamber 24 hours later were counted on tissue cross sections. Results: In all mice, EIU was characterised by a polymorphonuclear and mononuclear cell infiltrate. Numbers of infiltrating cells did not differ significantly between control and BLT1 gene knockout mice. Conclusion: Chemotactic factors other than LTB4 are primarily responsible for leukocyte migration into the eye during murine EIU.

Original language	English (US)
Pages (from-to)	273-275
Number of pages	3
Journal	British Journal of Ophthalmology
Volume	88
Issue number	2
DOIs	https://doi.org/10.1136/bjo.2003.027243
State	Published - Feb 2004

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

Access to Document

10.1136/bjo.2003.027243

Cite this

@article{ef7e2be18baa4233b111f9ef17316f77,

title = "Susceptibility to endotoxin induced uveitis is not reduced in mice deficient in BLT1, the high affinity leukotriene B4 receptor",

abstract = "Aim: To investigate the role of arachidonic acid derived chemotactic factor, LTB4, in the development of endotoxin induced uveitis (EIU), using mice deficient in the BLT1 gene which encodes the high affinity LTB4 receptor. Methods: BLT1 gene deficient and wild type BALB/c mice were injected intravitreally with Escherichia coli 055:B5 lipopolysaccharide (250 ng/2 μl). Number of leukocytes invading the anterior chamber 24 hours later were counted on tissue cross sections. Results: In all mice, EIU was characterised by a polymorphonuclear and mononuclear cell infiltrate. Numbers of infiltrating cells did not differ significantly between control and BLT1 gene knockout mice. Conclusion: Chemotactic factors other than LTB4 are primarily responsible for leukocyte migration into the eye during murine EIU.",

author = "Smith, {J. R.} and K. Subbarao and Franc, {D. T.} and B. Haribabu and Rosenbaum, {J. T.}",

year = "2004",

month = feb,

doi = "10.1136/bjo.2003.027243",

language = "English (US)",

volume = "88",

pages = "273--275",

journal = "British Journal of Ophthalmology",

issn = "0007-1161",

publisher = "BMJ Publishing Group",

number = "2",

}

TY - JOUR

T1 - Susceptibility to endotoxin induced uveitis is not reduced in mice deficient in BLT1, the high affinity leukotriene B4 receptor

AU - Smith, J. R.

AU - Subbarao, K.

AU - Franc, D. T.

AU - Haribabu, B.

AU - Rosenbaum, J. T.

PY - 2004/2

Y1 - 2004/2

N2 - Aim: To investigate the role of arachidonic acid derived chemotactic factor, LTB4, in the development of endotoxin induced uveitis (EIU), using mice deficient in the BLT1 gene which encodes the high affinity LTB4 receptor. Methods: BLT1 gene deficient and wild type BALB/c mice were injected intravitreally with Escherichia coli 055:B5 lipopolysaccharide (250 ng/2 μl). Number of leukocytes invading the anterior chamber 24 hours later were counted on tissue cross sections. Results: In all mice, EIU was characterised by a polymorphonuclear and mononuclear cell infiltrate. Numbers of infiltrating cells did not differ significantly between control and BLT1 gene knockout mice. Conclusion: Chemotactic factors other than LTB4 are primarily responsible for leukocyte migration into the eye during murine EIU.

AB - Aim: To investigate the role of arachidonic acid derived chemotactic factor, LTB4, in the development of endotoxin induced uveitis (EIU), using mice deficient in the BLT1 gene which encodes the high affinity LTB4 receptor. Methods: BLT1 gene deficient and wild type BALB/c mice were injected intravitreally with Escherichia coli 055:B5 lipopolysaccharide (250 ng/2 μl). Number of leukocytes invading the anterior chamber 24 hours later were counted on tissue cross sections. Results: In all mice, EIU was characterised by a polymorphonuclear and mononuclear cell infiltrate. Numbers of infiltrating cells did not differ significantly between control and BLT1 gene knockout mice. Conclusion: Chemotactic factors other than LTB4 are primarily responsible for leukocyte migration into the eye during murine EIU.

UR - http://www.scopus.com/inward/record.url?scp=0942290461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0942290461&partnerID=8YFLogxK

U2 - 10.1136/bjo.2003.027243

DO - 10.1136/bjo.2003.027243

M3 - Article

C2 - 14736790

AN - SCOPUS:0942290461

SN - 0007-1161

VL - 88

SP - 273

EP - 275

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

IS - 2

ER -

Susceptibility to endotoxin induced uveitis is not reduced in mice deficient in BLT1, the high affinity leukotriene B₄ receptor

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this