Survval of K+ permeability and gating currents in squid axons perfused with K+-free media

Wolfhard Almers, C. M. Armstrong

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

K+ currents were recorded in squid axons internally perfused with impermeant electrolyte. Total absence of permeant ions inside and out leads to an irreversible loss of potassium conductance with a time constant of ~ 11 min at 8° C. Potassium channels can be protected against this effect by external K+, Cs+, NH4 +, and Rb at concentrations of 100-440 mM. These experiments suggest that a K+ channel is normally occupied by 1 or more small cations, and becomes nonfunctional when these cations are removed. A large charge movement said to be related to K+ channel gating in frog skeletal muscle is absent in squid giant axons. However, deliberate destruction of K+ conductance by removal of permeant cations is accompanied by measurable loss to contain a contribution from K+ gating charge movements of more than 5 elementary charges per channel.

Original languageEnglish (US)
Pages (from-to)61-78
Number of pages18
JournalJournal of General Physiology
Volume75
Issue number1
StatePublished - 1980
Externally publishedYes

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Decapodiformes
Axons
Cations
Permeability
Potassium Channels
Anura
Electrolytes
Potassium
Skeletal Muscle
Ions

ASJC Scopus subject areas

  • Physiology

Cite this

Survval of K+ permeability and gating currents in squid axons perfused with K+-free media. / Almers, Wolfhard; Armstrong, C. M.

In: Journal of General Physiology, Vol. 75, No. 1, 1980, p. 61-78.

Research output: Contribution to journalArticle

Almers, Wolfhard ; Armstrong, C. M. / Survval of K+ permeability and gating currents in squid axons perfused with K+-free media. In: Journal of General Physiology. 1980 ; Vol. 75, No. 1. pp. 61-78.
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