Survivin expression in juvenile-onset recurrent respiratory papillomatosis

David M. Poetker, Anthony D. Sandler, David Scott, Richard J H Smith, Nancy M. Bauman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Recurrent respiratory papillomatosis (RRP), caused by the human papillomavirus, is characterized by unregulated growth of wartlike neoplasms on laryngeal mucosa. Apoptosis is important in normal cellular homeostasis, and dysregulation of this process is thought to govern the behavior of certain neoplasms. This study evaluates the expression of several pro-apoptotic and anti-apoptotic factors in papillomas of patients with RRP, with a specific interest in survivin, a cell cycle-regulated anti-apoptotic factor. Three antiapoptotic and 6 pro-apoptotic messenger RNA (mRNA) species were quantified by ribonuclease protection assay in 11 RRP papilloma specimens and 5 normal laryngeal specimens. Anti-apoptotic and pro-apoptotic mRNA ratios were quantified by normalizing to the ribosomal protein L32 and compared between specimens. Protein expression of survivin in tissue samples was also evaluated. The mean (±SD) expression of survivin was almost fivefold greater in the RRP papillomas than in normal tissue (14.2% ± 2.5% versus 3.0% ± 0.8% of L32, p = .003). The RRP specimens also had greater expression of XIAP, Fas, and p53 than did the normal tissue. Survivin protein was differentially expressed in the papilloma specimens, and was greatest in a papilloma that underwent malignant transformation. Survivin was absent in all normal laryngeal tissue tested. Apoptotic factors in general appear to be up-regulated in papillomatous tissue as compared to normal laryngeal tissue and may suggest a higher proliferation rate and cell turnover. Survivin is abundant in papillomas and absent in normal laryngeal tissue. Dysregulation of apoptosis as determined by abnormal expression of anti-apoptotic factors like survivin and XIAP probably favors papilloma growth and survival. Such factors may represent potential targets in the treatment of this disease.

Original languageEnglish (US)
Pages (from-to)957-961
Number of pages5
JournalAnnals of Otology, Rhinology and Laryngology
Volume111
Issue number11
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Papilloma
Laryngeal Mucosa
Apoptosis
Messenger RNA
Ribonucleases
Growth
Recurrent respiratory papillomatosis
Neoplasms
Cell Cycle
Proteins
Homeostasis
Cell Proliferation
Survival

Keywords

  • Apoptosis
  • Human papillomavirus
  • Recurrent respiratory papillomatosis
  • Survivin
  • XIAP

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Poetker, D. M., Sandler, A. D., Scott, D., Smith, R. J. H., & Bauman, N. M. (2002). Survivin expression in juvenile-onset recurrent respiratory papillomatosis. Annals of Otology, Rhinology and Laryngology, 111(11), 957-961.

Survivin expression in juvenile-onset recurrent respiratory papillomatosis. / Poetker, David M.; Sandler, Anthony D.; Scott, David; Smith, Richard J H; Bauman, Nancy M.

In: Annals of Otology, Rhinology and Laryngology, Vol. 111, No. 11, 01.11.2002, p. 957-961.

Research output: Contribution to journalArticle

Poetker, DM, Sandler, AD, Scott, D, Smith, RJH & Bauman, NM 2002, 'Survivin expression in juvenile-onset recurrent respiratory papillomatosis', Annals of Otology, Rhinology and Laryngology, vol. 111, no. 11, pp. 957-961.
Poetker, David M. ; Sandler, Anthony D. ; Scott, David ; Smith, Richard J H ; Bauman, Nancy M. / Survivin expression in juvenile-onset recurrent respiratory papillomatosis. In: Annals of Otology, Rhinology and Laryngology. 2002 ; Vol. 111, No. 11. pp. 957-961.
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