Survival of recombinant monoclonal antibodies (IgG, IgA and sIgA) versus naturally‐occurring antibodies (IgG and sIgA/IgA) in an ex vivo infant digestion model

Jiraporn Lueangsakulthai, Baidya Nath P. Sah, Brian P. Scottoline, David C. Dallas

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

To prevent infectious diarrhea in infants, orally‐supplemented enteric pathogen‐specific recombinant antibodies would need to resist degradation in the gastrointestinal tract. Palivizumab, a recombinant antibody specific to respiratory syncytial virus (RSV), was used as a model to assess the digestion of neutralizing antibodies in infant digestion. The aim was to determine the remaining binding activity of RSV F protein‐specific monoclonal and naturally‐occurring immunoglobulins (Ig) in different isoforms (IgG, IgA, and sIgA) across an ex vivo model of infant digestion. RSV F protein‐specific monoclonal immunoglobulins (IgG, IgA, and sIgA) and milk‐derived naturally-occurring Ig (IgG and sIgA/IgA) were exposed to an ex vivo model of digestion using digestive samples from infants (gastric and intestinal samples). The survival of each antibody was tested via an RSV F protein‐specific ELISA. Ex vivo gastric and intestinal digestion degraded palivizumab IgG, IgA, and sIgA (p < 0.05). However, the naturally‐occurring RSV F protein‐specific IgG and sIgA/IgA found in human milk were stable across gastric and intestinal ex vivo digestion. The structural differences between recombinant and naturally‐occurring antibodies need to be closely examined to guide future design of recombinant antibodies with increased stability for use in the gastrointestinal tract.

Original languageEnglish (US)
Article number621
JournalNutrients
Volume12
Issue number3
DOIs
StatePublished - Mar 2020

Keywords

  • Gastrointestinal digestion
  • Immunoglobulins
  • Infants
  • Palivizumab
  • Respiratory syncytial virus

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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