TY - JOUR
T1 - Surface morphology of human mononuclear phagocytes during maturation and phagocytosis
AU - Parakkal, P.
AU - Pinto, J.
AU - Hanifin, J. M.
N1 - Funding Information:
The first scanning electron microscope studies of cultured macrophages have for the most part confirmed the classic observations about their surface reliefs and 1 This work was supported in part by a Medical Research Foundation of Oregon Grant 728-28.06 to Dr. Jon M. Hanifin, Department of Dermatology, University of Oregon Medical School. This work was also supported in part by N1H Grant No. HD-04971 to Dr. Paul Parakkal and NIH Grant No. RR-00163 to the Oregon Regional Primate Research Center. 2 Publication No. 600 of the Oregon Regional Primate Research Center. 3 Present address: Division of Research Grants, N.I.H. Bethesda. MD.
PY - 1974/8
Y1 - 1974/8
N2 - Scanning electron micrographs revealed marked variations in surface morphology and size of mononuclear phagocytes cultured for short (4 hour) and long (7-45 days) durations. Soon after isolation from blood, monocytes showed only a few filopodia but extensive ruffled membranes. During culture, they increased greatly in size as they matured into macrophages. The macrophage surface showed large numbers of microvillous projections and elongated radiating filopodia. Intercommunication of filopodia between adjacent cells was observed. Addition of Candida spores and latex particles allowed sequential photographic documentation of the phagocytic process. Filopodia attached to the particle and subsequently became confluent to form a continuous membrane which enveloped the particle and eventually interiorized it. When a maximum number of particles had been engulfed, the macrophages appeared bloated with only a thin membrane, devoid of cytoplasmic extensions, surrounding the ingested particles.
AB - Scanning electron micrographs revealed marked variations in surface morphology and size of mononuclear phagocytes cultured for short (4 hour) and long (7-45 days) durations. Soon after isolation from blood, monocytes showed only a few filopodia but extensive ruffled membranes. During culture, they increased greatly in size as they matured into macrophages. The macrophage surface showed large numbers of microvillous projections and elongated radiating filopodia. Intercommunication of filopodia between adjacent cells was observed. Addition of Candida spores and latex particles allowed sequential photographic documentation of the phagocytic process. Filopodia attached to the particle and subsequently became confluent to form a continuous membrane which enveloped the particle and eventually interiorized it. When a maximum number of particles had been engulfed, the macrophages appeared bloated with only a thin membrane, devoid of cytoplasmic extensions, surrounding the ingested particles.
UR - http://www.scopus.com/inward/record.url?scp=0016297233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016297233&partnerID=8YFLogxK
U2 - 10.1016/S0022-5320(74)80078-X
DO - 10.1016/S0022-5320(74)80078-X
M3 - Article
C2 - 4602910
AN - SCOPUS:0016297233
SN - 0022-5320
VL - 48
SP - 216
EP - 226
JO - Journal of Ultrasructure Research
JF - Journal of Ultrasructure Research
IS - 2
ER -