Surface-enhanced resonance raman scattering spectroscopy applied to phytochrome and its model compounds. 2. Phytochrome and phycocyanin chromophorest

David L. Farrens, Randall E. Holt, Bernard N. Rospendowski, Pill Soon Song, Therese M. Cotton

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Surface-enhanced resonance Raman scattering (SERRS) spectra of phytochrome at 77 K are reported. The spectra reveal significant differences between Pr and Pfr forms of phytochrome. SERRS spectra of C-phycocyanin Z, Z, Z- and Z, Z, E-chromopeptide isomers at 77 K are also reported. The phycocyanin chromopeptide studies are used to provide a basis for interpreting the phytochrome SERRS spectra. The spectra indicate that photoisomerization of chromophores from C-phycocyanin chromopeptides (from a Z, Z, Z to a Z, Z, E configuration) is detectable with SERRS. Comparison of SERRS spectra between the Pr and Pfr forms of 124-kDa phytochrome adsorbed on silver colloids demonstrates that the chromophore undergoes a Z →E isomerization during the Pr →Pfr phototransformation. However, the overall chromophore conformations are likely to be conserved for the native Pr and Pfr phytochrome species.

Original languageEnglish (US)
Pages (from-to)9162-9169
Number of pages8
JournalJournal of the American Chemical Society
Volume111
Issue number26
DOIs
StatePublished - Dec 1989

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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