Surface charge properties of and Cu(II) adsorption by spores of the marine Bacillus sp. strain SG-1

M. Lee He, Bradley Tebo

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Abstract

Spores of marine Bacillus sp. strain SG-1 are capable of oxidizing Mn(II) and Co(II), which results in the precipitation of Mn(III, IV) and Co(III) oxides and hydroxides on the spore surface. The spores also bind other heavy metals; however, little is known about the mechanism and capacity of this metal binding. In this study the characteristics of the spore surface and Cu(II) adsorption to this surface were investigated. The specific surface area of wet SG-1 spores was 74.7 m2 per g of dry weight as measured by the methylene blue adsorption method. This surface area is 11-fold greater than the surface area of dried spores, as determined with an N2 adsorption surface area analyzer or as calculated from the spore dimensions, suggesting that the spore surface is porous. The surface exchange capacity as measured by the proton exchange method was found to be 30.6 μmol m-2, which is equal to a surface site density of 18.3 sites nm-2. The SG-1 spore surface charge characteristics were obtained from acid-base titration data. The surface charge density varied with pH, and the zero point of charge was pH 4.5. The titration curves suggest that the spore surface is dominated by negatively charged sites that are largely carboxylate groups but also phosphate groups. Copper adsorption by SG-1 spores was rapid and complete within minutes. The spores exhibited a high affinity for Cu(II). The amounts of copper adsorbed increased from negligible at pH 3 to maximum levels at pH >6. Their great surface area, site density, and affinity give SG-1 spores a high capability for binding metals on their surfaces, as demonstrated by our experiments with Cu(II).

Original languageEnglish (US)
Pages (from-to)1123-1129
Number of pages7
JournalApplied and Environmental Microbiology
Volume64
Issue number3
StatePublished - Mar 1998
Externally publishedYes

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Surface Properties
Spores
Bacillus
Adsorption
spore
adsorption
spores
surface area
metal binding
titration
Copper
copper
Metals
Hydroxides
charge characteristics
metals
phosphate group
hydroxides
methylene blue
Methylene Blue

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Surface charge properties of and Cu(II) adsorption by spores of the marine Bacillus sp. strain SG-1. / He, M. Lee; Tebo, Bradley.

In: Applied and Environmental Microbiology, Vol. 64, No. 3, 03.1998, p. 1123-1129.

Research output: Contribution to journalArticle

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