Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy

Benjamin W. Neuman, Brian D. Adair, Craig Yoshioka, Joel D. Quispe, Gretchen Orca, Peter Kuhn, Ronald A. Milligan, Mark Yeager, Michael J. Buchmeier

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Coronavirus particles are enveloped and pleomorphic and are thus refractory to crystallization and symmetry-assisted reconstruction. A novel methodology of single-particle image analysis was applied to selected virus features to obtain a detailed model of the oligomeric state and spatial relationships among viral structural proteins. Two-dimensional images of the S, M, and N structural proteins of severe acute respiratory syndrome coronavirus and two other coronaviruses were refined to a resolution of ~4 nm. Proteins near the viral membrane were arranged in overlapping lattices surrounding a disordered core. Trimeric glycoprotein spikes were in register with four underlying ribonucleoprotein densities. However, the spikes were dispensable for ribonucleoprotein lattice formation. The ribonucleoprotein particles displayed coiled shapes when released from the viral membrane. Our results contribute to the understanding of the assembly pathway used by coronaviruses and other pieomorpliic viruses and provide the first detailed view of coronavirus ultrastructure.

Original languageEnglish (US)
Pages (from-to)7918-7928
Number of pages11
JournalJournal of Virology
Volume80
Issue number16
DOIs
StatePublished - Aug 2006
Externally publishedYes

Fingerprint

Cryoelectron Microscopy
Severe Acute Respiratory Syndrome
Coronavirus
Coronavirinae
ribonucleoproteins
Ribonucleoproteins
structural proteins
viruses
Viral Matrix Proteins
viral proteins
Viruses
Viral Structural Proteins
crystallization
glycoproteins
ultrastructure
Crystallization
image analysis
Glycoproteins
cryo-electron microscopy
Severe acute respiratory syndrome coronavirus

ASJC Scopus subject areas

  • Immunology

Cite this

Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy. / Neuman, Benjamin W.; Adair, Brian D.; Yoshioka, Craig; Quispe, Joel D.; Orca, Gretchen; Kuhn, Peter; Milligan, Ronald A.; Yeager, Mark; Buchmeier, Michael J.

In: Journal of Virology, Vol. 80, No. 16, 08.2006, p. 7918-7928.

Research output: Contribution to journalArticle

Neuman, BW, Adair, BD, Yoshioka, C, Quispe, JD, Orca, G, Kuhn, P, Milligan, RA, Yeager, M & Buchmeier, MJ 2006, 'Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy', Journal of Virology, vol. 80, no. 16, pp. 7918-7928. https://doi.org/10.1128/JVI.00645-06
Neuman, Benjamin W. ; Adair, Brian D. ; Yoshioka, Craig ; Quispe, Joel D. ; Orca, Gretchen ; Kuhn, Peter ; Milligan, Ronald A. ; Yeager, Mark ; Buchmeier, Michael J. / Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy. In: Journal of Virology. 2006 ; Vol. 80, No. 16. pp. 7918-7928.
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