Suppression of TRPM7 Inhibits Proliferation, Migration, and Invasion of Malignant Human Glioma Cells

Tian Dong Leng, Ming Hua Li, Jian Feng Shen, Ming Li Liu, Xin Bo Li, Hua Wei Sun, Debbie Branigan, Zhao Zeng, Hong Fang Si, Jun Li, Jeff Chen, Zhi Gang Xiong

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Summary: Background: Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood, and no effective treatment has been developed for GBM patients. Aims: We determine the role of TRPM7 channels in the growth, migration, and infiltration of malignant glioma cells. Methods: Using a combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated the expression of functional TRPM7 channels of A172 cells, a human glioma cell line, as well as in human glioma tissues. Furthermore, we evaluated the role of TRPM7 in growth, migration, and infiltration of A172 cells with MTT and transwell migration and invasion assays. Results: We showed the expression of functional TRPM7 channels in both A172 cells and human glioma tissues. Suppression of TRPM7 expression with TRPM7-siRNA dramatically reduced the proliferation, migration, and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with 2-aminoethoxydiphenyl borate (2-APB) showed a similar effect as TRPM7-siRNA. Conclusion: We demonstrate that human glioma cells express functional TRPM7 channel and that activation of this channel plays an important role in the proliferation, migration, and invasion of malignant glioma cells. TRPM7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.

Original languageEnglish (US)
Pages (from-to)252-261
Number of pages10
JournalCNS Neuroscience and Therapeutics
Volume21
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • Glioma
  • Invasion
  • Migration
  • Proliferation
  • TRPM7

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health
  • Physiology (medical)
  • Pharmacology (medical)

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