56Fe Irradiation Alters Spine Density and Dendritic Complexity in the Mouse Hippocampus

Antiño R. Allen, Jacob Raber, Ayanabha Chakraborti, Sourabh Sharma, John R. Fike

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A unique feature of the space radiation environment is the presence of high-energy charged particles, which can be significantly hazardous to space flight crews who are exposed during a mission. Health risks associated with high-LET radiation exposure include cognitive injury. The pathogenesis of this injury is unknown but may involve modifications to dendritic structure and/or alterations in dendritic spine density and morphology. In this study, 24 two-month-old C57BL6/J male mice were either whole-body irradiated with 0.5 Gy 56Fe (600 MeV/n; n = 12) or sham irradiated (n = 12). Three months postirradiation animals were tested for locomotor activity and habituation. After behavioral testing, animals were euthanized and the brains were flash frozen. Compared to sham-irradiated mice, irradiated mice moved less when first introduced to the environment, although they did recognize the environment when re-exposed to it one day later. Exposure to 56Fe radiation significantly compromised the dendritic architecture and reduced spine density throughout the hippocampal tri-synaptic network. To our knowledge, these data represents the first reported evidence that high-LET radiation has deleterious effects on mature neurons associated with hippocampal learning and memory.

Original languageEnglish (US)
Pages (from-to)586-594
Number of pages9
JournalRadiation Research
Volume184
Issue number6
DOIs
StatePublished - Dec 1 2015

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hippocampus
Dendritic Spines
spine
mice
Linear Energy Transfer
Hippocampus
Radiation
irradiation
animals
flight crews
Space Flight
pathogenesis
extraterrestrial radiation
space flight
Wounds and Injuries
radiation dosage
radiation
Locomotion
neurons
learning

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

56Fe Irradiation Alters Spine Density and Dendritic Complexity in the Mouse Hippocampus. / Allen, Antiño R.; Raber, Jacob; Chakraborti, Ayanabha; Sharma, Sourabh; Fike, John R.

In: Radiation Research, Vol. 184, No. 6, 01.12.2015, p. 586-594.

Research output: Contribution to journalArticle

Allen, Antiño R. ; Raber, Jacob ; Chakraborti, Ayanabha ; Sharma, Sourabh ; Fike, John R. / 56Fe Irradiation Alters Spine Density and Dendritic Complexity in the Mouse Hippocampus. In: Radiation Research. 2015 ; Vol. 184, No. 6. pp. 586-594.
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