23Na-NMR measurements of intracellular sodium in intact perfused ferret hearts during ischemia and reperfusion

M. M. Pike, M. Kitakaze, E. Marban

Research output: Contribution to journalArticle

134 Scopus citations

Abstract

23Na nuclear magnetic resonance (NMR) spectroscopy was utilized to measure intracellular Na+ in perfused ferret hearts exposed to the shift reagent dysprosium triethylenetetramine-hexa-acetic acid [Dy(TTHA)3-]. The intracellular Na+ signal was small under normal perfusion conditions; resolution was enhanced by using a Jump-Return NMR pulse protocol. During 20 min of total global ischemia at 30°C, intracellular Na+ concentration ([Na+](i)) increased steadily to a peak value fivefold greater than control. [Na+](i) declined monotonically back to control levels within 9 min of reperfusion. In contrast, the mean contractile pressure only recovered to 54% of control levels. Thus major alterations in Na+ homeostasis occur during severe ischemia. [Na+] recovers rapidly during reperfusion and is therefore dissociated from the lingering postischemic depression of contractile function known as 'stunning'.

Original languageEnglish (US)
Pages (from-to)H1767-H1773
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume259
Issue number6 28-6
StatePublished - Dec 1 1990

Keywords

  • cardiac function
  • nuclear magnetic resonance spectroscopy
  • stunned myocardium

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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