13C-lutein is differentially distributed in tissues of an adult female rhesus macaque following a single oral administration: a pilot study

Sookyoung Jeon, Qiyao Li, Stanislav S. Rubakhin, Jonathan V. Sweedler, Joshua W. Smith, Martha Neuringer, Matthew Kuchan, John W. Erdman

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Despite the growing awareness regarding lutein's putative roles in eyes and brain, its pharmacokinetics and tissue distribution in primates have been poorly understood. We hypothesized that 13C-lutein will be differentially distributed into tissues of an adult rhesus macaque (Macaca mulatta) 3 days following a single oral dose. After a year of prefeeding a diet supplemented with unlabeled lutein (1 μmol/kg/d), a 19-year-old female was dosed with 1.92 mg of highly enriched 13C-lutein. Tissues of a nondosed, lutein-fed monkey were used as a reference for natural abundance of 13C-lutein. On the third day postdose, plasma and multiple tissues were collected. Lutein was quantified by high-performance liquid chromatography–photodiode array detector, and 13C-lutein tissue enrichment was determined by liquid chromatography quadrupole time-of-flight mass spectrometry. In the tissues of a reference monkey, 12C-lutein with natural abundance of 13C-lutein was detectable. In the dosed monkey, highly enriched 13C-lutein was observed in all analyzed tissues except for the macular and peripheral retina, with the highest concentrations in the liver followed by the adrenal gland and plasma. 13C-lutein accumulated differentially across 6 brain regions. In adipose depots, 13C-lutein was observed, with the highest concentrations in the axillary brown adipose tissues. In summary, we evaluated 13C-lutein tissue distribution in a nonhuman primate following a single dose of isotopically labeled lutein. These results show that tissue distribution 3 days following a dose of lutein varied substantially dependent on tissue type.

    Original languageEnglish (US)
    JournalNutrition Research
    DOIs
    StateAccepted/In press - Jan 1 2018

    Fingerprint

    Lutein
    Macaca mulatta
    Oral Administration
    Tissue Distribution
    Haplorhini
    Primates
    Brown Adipose Tissue
    Brain
    Adrenal Glands

    Keywords

    • Biodistribution
    • Isotopic tracer
    • Lutein
    • Mass spectrometry
    • Rhesus macaque

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Endocrinology
    • Nutrition and Dietetics

    Cite this

    13C-lutein is differentially distributed in tissues of an adult female rhesus macaque following a single oral administration : a pilot study. / Jeon, Sookyoung; Li, Qiyao; Rubakhin, Stanislav S.; Sweedler, Jonathan V.; Smith, Joshua W.; Neuringer, Martha; Kuchan, Matthew; Erdman, John W.

    In: Nutrition Research, 01.01.2018.

    Research output: Contribution to journalArticle

    Jeon, Sookyoung ; Li, Qiyao ; Rubakhin, Stanislav S. ; Sweedler, Jonathan V. ; Smith, Joshua W. ; Neuringer, Martha ; Kuchan, Matthew ; Erdman, John W. / 13C-lutein is differentially distributed in tissues of an adult female rhesus macaque following a single oral administration : a pilot study. In: Nutrition Research. 2018.
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    abstract = "Despite the growing awareness regarding lutein's putative roles in eyes and brain, its pharmacokinetics and tissue distribution in primates have been poorly understood. We hypothesized that 13C-lutein will be differentially distributed into tissues of an adult rhesus macaque (Macaca mulatta) 3 days following a single oral dose. After a year of prefeeding a diet supplemented with unlabeled lutein (1 μmol/kg/d), a 19-year-old female was dosed with 1.92 mg of highly enriched 13C-lutein. Tissues of a nondosed, lutein-fed monkey were used as a reference for natural abundance of 13C-lutein. On the third day postdose, plasma and multiple tissues were collected. Lutein was quantified by high-performance liquid chromatography–photodiode array detector, and 13C-lutein tissue enrichment was determined by liquid chromatography quadrupole time-of-flight mass spectrometry. In the tissues of a reference monkey, 12C-lutein with natural abundance of 13C-lutein was detectable. In the dosed monkey, highly enriched 13C-lutein was observed in all analyzed tissues except for the macular and peripheral retina, with the highest concentrations in the liver followed by the adrenal gland and plasma. 13C-lutein accumulated differentially across 6 brain regions. In adipose depots, 13C-lutein was observed, with the highest concentrations in the axillary brown adipose tissues. In summary, we evaluated 13C-lutein tissue distribution in a nonhuman primate following a single dose of isotopically labeled lutein. These results show that tissue distribution 3 days following a dose of lutein varied substantially dependent on tissue type.",
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    AU - Rubakhin, Stanislav S.

    AU - Sweedler, Jonathan V.

    AU - Smith, Joshua W.

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