125I-Luteinizing Hormone (LH) binding to soluble receptors from the primate (Macaca mulatta) corpus luteum: Effects of ethanol exposure

Douglas R. Danforth, Richard Stouffer

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    Abstract

    In vitro exposure to alcohols unmasks additional binding sites for gonadotropin in cell/membrane preparations of the corpus luteum of rhesus monkeys. In the current study, we compared the effects of ethanol on gonadotropin receptors solubilized from macaque luteal membranes to those on receptors associated with the lipid bilayer. Treatment with 1% Triton X-100 for 30m min at 4C, followed by precipitation with polyethylene glycol, resulted in recovery of 50% more binding sites for 125I-human luteinizing hormone (hLH) than were available in particulate preparations (p125I-hLH (p125I-hLH binding to soluble receptors. Steady-state LH binding to soluble receptors during incubation at 4C was half of that observed at 25C. The presence of 8% ethanol at 4C restored LH binding to levels observed in the absence of ethanol at 25C. Thus, LH binding sites in the primate corpus luteum can be effectively solubilized with Triton X-100. The different binding characteristics of particulate and soluble receptors, including the response to ethanol exposure, suggest that the lipid environment in the luteal membrane modulates the availability and affinity of gonadotropin receptors.

    Original languageEnglish (US)
    Pages (from-to)635-642
    Number of pages8
    JournalLife Sciences
    Volume43
    Issue number7
    DOIs
    StatePublished - 1988

    Fingerprint

    Corpus Luteum
    Luteinizing Hormone
    Macaca mulatta
    Primates
    Ethanol
    Gonadotropin Receptors
    Binding Sites
    Octoxynol
    Membranes
    Lipid bilayers
    Lipid Bilayers
    Macaca
    Cell membranes
    Gonadotropins
    Alcohols
    Cell Membrane
    Availability
    Lipids
    Recovery

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    125I-Luteinizing Hormone (LH) binding to soluble receptors from the primate (Macaca mulatta) corpus luteum : Effects of ethanol exposure. / Danforth, Douglas R.; Stouffer, Richard.

    In: Life Sciences, Vol. 43, No. 7, 1988, p. 635-642.

    Research output: Contribution to journalArticle

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    abstract = "In vitro exposure to alcohols unmasks additional binding sites for gonadotropin in cell/membrane preparations of the corpus luteum of rhesus monkeys. In the current study, we compared the effects of ethanol on gonadotropin receptors solubilized from macaque luteal membranes to those on receptors associated with the lipid bilayer. Treatment with 1{\%} Triton X-100 for 30m min at 4C, followed by precipitation with polyethylene glycol, resulted in recovery of 50{\%} more binding sites for 125I-human luteinizing hormone (hLH) than were available in particulate preparations (p125I-hLH (p125I-hLH binding to soluble receptors. Steady-state LH binding to soluble receptors during incubation at 4C was half of that observed at 25C. The presence of 8{\%} ethanol at 4C restored LH binding to levels observed in the absence of ethanol at 25C. Thus, LH binding sites in the primate corpus luteum can be effectively solubilized with Triton X-100. The different binding characteristics of particulate and soluble receptors, including the response to ethanol exposure, suggest that the lipid environment in the luteal membrane modulates the availability and affinity of gonadotropin receptors.",
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