Sulforaphane bioavailability and chemopreventive activity in women scheduled for breast biopsy

Lauren L. Atwell, Zhenzhen Zhang, Motomi (Tomi) Mori, Paige E. Farris, John Vetto, Arpana Naik, Karen Oh, Philippe Thuillier, Emily Ho, Jackilen (Jackie) Shannon

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Epidemiologic studies suggest a protective effect of cruciferous vegetables on breast cancer. Sulforaphane (SFN), an active food component derived from crucifers, has been shown to be effective in breast cancer chemoprevention. This study evaluated the chemopreventive effect of SFN on selective biomarkers from blood and breast tissues. In a 2- To 8-week double-blinded, randomized controlled trial, 54 women with abnormal mammograms and scheduled for breast biopsy were randomized to consume a placebo or a glucoraphanin (GFN) supplement providing SFN (n = 27). Plasma and urinary SFN metabolites, peripheral blood mononuclear cell (PBMC) histone deacetylase (HDAC) activity, and tissue biomarkers (H3K18ac, H3K9ac, HDAC3, HDAC6, Ki- 67, p21) were measured before and after the intervention in benign, ductal carcinoma in situ, or invasive ductal carcinoma breast tissues. Within the supplement group, Ki-67 (P = 0.003) and HDAC3 (P = 0.044) levels significantly decreased in benign tissue. Pre-to-postintervention changes in these biomarkers were not significantly different between treatment groups after multiple comparison adjustment. GFN supplementation was associated with a significant decrease in PBMC HDAC activity (P = 0.04).Nosignificant associations were observed between SFN and examined tissue biomarkers when comparing treatment groups. This study provides evidence that GFN supplementation for a few weeks is safe but may not be sufficient for producing changes in breast tissue tumor biomarkers. Future studies employing larger sample sizes should evaluate alternative dosing and duration regimens to inform dietary SFN strategies in breast cancer chemoprevention.

Original languageEnglish (US)
Pages (from-to)1184-1191
Number of pages8
JournalCancer Prevention Research
Volume8
Issue number12
DOIs
StatePublished - Dec 1 2015

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Biological Availability
Breast
Biopsy
Biomarkers
Breast Neoplasms
Histone Deacetylases
Chemoprevention
Blood Cells
Carcinoma, Ductal, Breast
Carcinoma, Intraductal, Noninfiltrating
Tumor Biomarkers
Vegetables
Sample Size
sulforafan
Epidemiologic Studies
Randomized Controlled Trials
Placebos
Food
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sulforaphane bioavailability and chemopreventive activity in women scheduled for breast biopsy. / Atwell, Lauren L.; Zhang, Zhenzhen; Mori, Motomi (Tomi); Farris, Paige E.; Vetto, John; Naik, Arpana; Oh, Karen; Thuillier, Philippe; Ho, Emily; Shannon, Jackilen (Jackie).

In: Cancer Prevention Research, Vol. 8, No. 12, 01.12.2015, p. 1184-1191.

Research output: Contribution to journalArticle

Atwell, Lauren L. ; Zhang, Zhenzhen ; Mori, Motomi (Tomi) ; Farris, Paige E. ; Vetto, John ; Naik, Arpana ; Oh, Karen ; Thuillier, Philippe ; Ho, Emily ; Shannon, Jackilen (Jackie). / Sulforaphane bioavailability and chemopreventive activity in women scheduled for breast biopsy. In: Cancer Prevention Research. 2015 ; Vol. 8, No. 12. pp. 1184-1191.
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