Sulfated glycans stimulate endocytosis of the cellular isoform of the prion protein, PrPC, in cultured cells

Show-Ling Shyng, Sylvain Lehmann, Krista L. Moulder, David A. Harris

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

There is currently no effective therapy for human prion diseases. However, several polyanionic glycans, including pentosan sulfate and dextran sulfate, prolong the incubation time of scrapie in rodents inhibit the production of the scrapie isoform of the prion protein (PrPSc), the major component of infectious prions, in cultured neuroblastoma cells. We report here that pentosan sulfate and related compounds rapidly and dramatically reduce the amount of PrPC, the non-infectious precursor of PrPSc, present on the cell surface. This effect results primarily from the ability of these agents to stimulate endocytosis of PrPC, thereby causing a redistribution of the protein from the plasma membrane to the cell interior. Pentosan sulfate also causes a change in the ultrastructural localization of PrPC, such that a portion of the protein molecules are shifted into late endosomes and/or lysosomes. In addition, we demonstrate, using PrP-containing bacterial fusion proteins, that cultured cells express saturable and specific surface binding sites for PrP, many of which are glycosaminoglycan molecules. Our results raise the possibility that sulfated glycans inhibit prion production by altering the cellular localization of PrPC precursor, and they indicate that endogenous proteoglycans are likely to play an important role in the cellular metabolism of both PrPC and PrPSc.

Original languageEnglish (US)
Pages (from-to)30221-30229
Number of pages9
JournalJournal of Biological Chemistry
Volume270
Issue number50
StatePublished - Dec 15 1995
Externally publishedYes

Fingerprint

PrPC Proteins
Prions
Endocytosis
Sulfates
Polysaccharides
Scrapie
Cultured Cells
Protein Isoforms
Cells
PrPSc Proteins
Dextran Sulfate
Prion Diseases
Molecules
Bacterial Proteins
Endosomes
Proteoglycans
Cell membranes
Lysosomes
Glycosaminoglycans
Neuroblastoma

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sulfated glycans stimulate endocytosis of the cellular isoform of the prion protein, PrPC, in cultured cells. / Shyng, Show-Ling; Lehmann, Sylvain; Moulder, Krista L.; Harris, David A.

In: Journal of Biological Chemistry, Vol. 270, No. 50, 15.12.1995, p. 30221-30229.

Research output: Contribution to journalArticle

Shyng, Show-Ling ; Lehmann, Sylvain ; Moulder, Krista L. ; Harris, David A. / Sulfated glycans stimulate endocytosis of the cellular isoform of the prion protein, PrPC, in cultured cells. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 50. pp. 30221-30229.
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